Research output: Contribution to journal › Journal article › Research › peer-review
P URPOSE . To examine how circulating immune mediators in vivo may affect gene and protein expression at the RPE/choroid interface. M ETHODS . Young mice were systemically infected with lymphocytic choriomeningitis virus (LCMV) or continuously infused with IFN-γ. RPE/choroid was isolated and analyzed with whole-transcriptome gene expression microarrays. Selected gene expression findings were validated at the protein level. R ESULTS . Both the systemic immune activation from virus infection and the sterile systemically increased level of IFN-γ resulted in increased expression of chemokine ligands, chemokine receptors, and early complement components in isolates of RPE/choroid. These findings were largely absent from LCMV-infected mice deficient in either the interferon α/β receptor or IFN-γ. C ONCLUSIONS . Together, these findings demonstrate that acute systemic immune activation results in a local response at the RPE/choroid interface that may include chemokine-dependent recruitment of inflammatory cells and engagement of the complement system. This may represent a link between the systemic low-grade inflammation and the retinal pathology observed in several multifactorial entities such as aging, AMD, and diabetes.
Original language | English |
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Journal | Investigative Ophthalmology and Visual Science |
Volume | 60 |
Issue number | 1 |
Pages (from-to) | 192-201 |
Number of pages | 10 |
ISSN | 0146-0404 |
DOIs | |
Publication status | Published - 1 Jan 2019 |
ID: 59475635