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Chemokine expression in murine RPE/choroid in response to systemic viral infection and elevated levels of circulating interferon-γ

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P URPOSE . To examine how circulating immune mediators in vivo may affect gene and protein expression at the RPE/choroid interface. M ETHODS . Young mice were systemically infected with lymphocytic choriomeningitis virus (LCMV) or continuously infused with IFN-γ. RPE/choroid was isolated and analyzed with whole-transcriptome gene expression microarrays. Selected gene expression findings were validated at the protein level. R ESULTS . Both the systemic immune activation from virus infection and the sterile systemically increased level of IFN-γ resulted in increased expression of chemokine ligands, chemokine receptors, and early complement components in isolates of RPE/choroid. These findings were largely absent from LCMV-infected mice deficient in either the interferon α/β receptor or IFN-γ. C ONCLUSIONS . Together, these findings demonstrate that acute systemic immune activation results in a local response at the RPE/choroid interface that may include chemokine-dependent recruitment of inflammatory cells and engagement of the complement system. This may represent a link between the systemic low-grade inflammation and the retinal pathology observed in several multifactorial entities such as aging, AMD, and diabetes.

Original languageEnglish
JournalInvestigative Ophthalmology and Visual Science
Volume60
Issue number1
Pages (from-to)192-201
Number of pages10
ISSN0146-0404
DOIs
Publication statusPublished - 1 Jan 2019

    Research areas

  • Chemokine, Complement, Interferon, LCMV, Retina, RPE

ID: 59475635