Characterization of Rotavirus Strains in a Danish Population: High Frequency of Mixed Infections and Diversity within the VP4 Gene of P[8] Strains

T K Fischer, J Eugen-Olsen, Anne Grethe Julius Pedersen, K Mølbak, B Böttiger, Katrine Rostgaard, N M Nielsen

36 Citations (Scopus)

Abstract

We characterized the G and P types from 162 rotavirus-positive stool specimens collected from 162 persons in Denmark (134 children and 28 adults) with acute diarrhea in 1998, 2000, and 2002. Samples were obtained during outpatient consultations (73%) and from hospitalized patients (27%). Although more than 20 different G-P combinations were identified, only 52% represented the globally most common types G1P[8], G2P[4], and G4P[8]. The G9 genotype, which is emerging worldwide, was identified in 12% of all samples. Twenty-one percent of the samples were of mixed genotypic origin, which is the highest frequency reported in any European population. The standard reverse transcription-PCR methods initially failed to identify a considerable fraction of the rotavirus P strains due to mutations at the VP4 primer-binding sites of P[8] strains. The application of a degenerate P[8] primer resulted in typing of most VP4 strains. There was considerable year-to-year variation among the circulating G-P types, and whereas G1P[8] was predominant in 1998 (42% of samples) and 2002 (26%), G2P[4] was the strain that was most frequently detected in 2000 (26% of samples). Our findings might implicate challenges for rotavirus vaccine implementation in a European population and underscore the importance of extensive strain surveillance prior to, during, and after introduction of any vaccine candidate.
Original languageEnglish
JournalJournal of Clinical Microbiology
Volume43
Issue number3
Pages (from-to)1099-1104
Number of pages6
ISSN0095-1137
DOIs
Publication statusPublished - 2005

Keywords

  • Adolescent
  • Adult
  • Aged
  • Base Sequence
  • Capsid Proteins
  • Child
  • Child, Preschool
  • Female
  • Genotype
  • Humans
  • Infant
  • Infant, Newborn
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Reassortant Viruses
  • Rotavirus
  • Rotavirus Infections

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