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Characterization of cerebrospinal fluid by comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry

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@article{0552d02aaa0f49a89cd621179e136fcc,
title = "Characterization of cerebrospinal fluid by comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry",
abstract = "Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC×GC-TOFMS) was applied in the quantification and identification of organic compounds in patient-matched human cerebrospinal fluid (CSF) and serum samples. Concentrations of 21 amino and hydroxyl acids varied from 0.04 to 77ng/μl in CSF and from 0.1 to 84ng/μl in serum. In total, 91 metabolites out of over 1200 detected were identified based on mass spectra and retention indices. The other metabolites were identified at the functional group level. The main metabolites detected in CSF were sugar and amino acid derivatives. The CSF and serum had clearly distinct metabolic profiles, with larger biological variation in the serum than in CSF. The GC×GC-TOFMS allowed detection and identification of several metabolites that have not been previously detected in CSF.",
keywords = "Aged, Amino Acids/blood, Carbohydrates/blood, Female, Gas Chromatography-Mass Spectrometry/methods, Humans, Lipids/blood, Male, Metabolome, Reproducibility of Results",
author = "Minna Hartonen and Ismo Mattila and Anna-Liisa Ruskeep{\"a}{\"a} and Matej Orešič and Tuulia Hy{\"o}tyl{\"a}inen",
note = "Copyright {\circledC} 2013 Elsevier B.V. All rights reserved.",
year = "2013",
month = "6",
day = "7",
doi = "10.1016/j.chroma.2013.04.005",
language = "English",
volume = "1293",
pages = "142--9",
journal = "Journal of Chromatography",
issn = "0021-9673",
publisher = "Elsevier BV",

}

RIS

TY - JOUR

T1 - Characterization of cerebrospinal fluid by comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry

AU - Hartonen, Minna

AU - Mattila, Ismo

AU - Ruskeepää, Anna-Liisa

AU - Orešič, Matej

AU - Hyötyläinen, Tuulia

N1 - Copyright © 2013 Elsevier B.V. All rights reserved.

PY - 2013/6/7

Y1 - 2013/6/7

N2 - Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC×GC-TOFMS) was applied in the quantification and identification of organic compounds in patient-matched human cerebrospinal fluid (CSF) and serum samples. Concentrations of 21 amino and hydroxyl acids varied from 0.04 to 77ng/μl in CSF and from 0.1 to 84ng/μl in serum. In total, 91 metabolites out of over 1200 detected were identified based on mass spectra and retention indices. The other metabolites were identified at the functional group level. The main metabolites detected in CSF were sugar and amino acid derivatives. The CSF and serum had clearly distinct metabolic profiles, with larger biological variation in the serum than in CSF. The GC×GC-TOFMS allowed detection and identification of several metabolites that have not been previously detected in CSF.

AB - Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC×GC-TOFMS) was applied in the quantification and identification of organic compounds in patient-matched human cerebrospinal fluid (CSF) and serum samples. Concentrations of 21 amino and hydroxyl acids varied from 0.04 to 77ng/μl in CSF and from 0.1 to 84ng/μl in serum. In total, 91 metabolites out of over 1200 detected were identified based on mass spectra and retention indices. The other metabolites were identified at the functional group level. The main metabolites detected in CSF were sugar and amino acid derivatives. The CSF and serum had clearly distinct metabolic profiles, with larger biological variation in the serum than in CSF. The GC×GC-TOFMS allowed detection and identification of several metabolites that have not been previously detected in CSF.

KW - Aged

KW - Amino Acids/blood

KW - Carbohydrates/blood

KW - Female

KW - Gas Chromatography-Mass Spectrometry/methods

KW - Humans

KW - Lipids/blood

KW - Male

KW - Metabolome

KW - Reproducibility of Results

U2 - 10.1016/j.chroma.2013.04.005

DO - 10.1016/j.chroma.2013.04.005

M3 - Journal article

VL - 1293

SP - 142

EP - 149

JO - Journal of Chromatography

JF - Journal of Chromatography

SN - 0021-9673

ER -

ID: 54986325