Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Changes in P2Y6 receptor-mediated vasoreactivity following focal and global ischemia

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Gravity and lymphodynamics

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Spontaneous contractions of the human thoracic duct-Important for securing lymphatic return during positive pressure ventilation?

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Reliability of cerebral autoregulation using different measures of perfusion pressure in patients with subarachnoid hemorrhage

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Mutual stimulatory signaling between human myogenic cells and rat cerebellar neurons

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Repair-related molecular changes during recovery phase of ischemic stroke in female rats

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Ny medicin beskytter hjernen ved voldsomme hjerneblødninger hos rotter og hunde - nu bliver den testet på danske patienter

    Research output: Contribution to journalContribution to newspaper - Newspaper articleCommunication

  3. Dual action of the cannabinoid receptor 1 ligand arachidonyl-2'-chloroethylamide on calcitonin gene-related peptide release

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Lasmiditan and 5-Hydroxytryptamine in the rat trigeminal system; expression, release and interactions with 5-HT1 receptors

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Advances in migraine and headache therapy (BJP 75th anniversary)

    Research output: Contribution to journalEditorialResearchpeer-review

View graph of relations

Ischemia, both in the form of focal thromboembolic stroke and following subarachnoid hemorrhage (SAH), causes upregulation of vasoconstrictive receptor systems within the cerebral vasculature. Descriptions regarding changes in purinergic signaling following ischemia are lacking, especially when the importance of purinergic signaling in regulating vascular tone is taken into consideration. This prompted us to evaluate changes in P2Y6 -mediated vasomotor reactivity in two different stroke models in rat. We used wire myography to measure changes in cerebral vasoreactivity to the P2Y6 agonist UDP-β-S following either experimental SAH or transient middle cerebral artery occlusion. Changes in receptor localization or receptor expression were evaluated using immunohistochemistry and quantitative flow cytometry. Transient middle cerebral artery occlusion caused an increase in Emax when compared to sham (233.6 [206.1-258.5]% vs. 161.1 [147.1-242.6]%, p = 0.0365). No such change was seen following SAH. Both stroke models were associated with increased levels of P2Y6 receptor expression in the vascular smooth muscle cells (90.94 [86.99-99.15]% and 93.79 [89.96-96.39]% vs. 80.31 [70.80-80.86]%, p = 0.021) and p = 0.039 respectively. There was no change in receptor localization in either of the stroke models. Based on these findings, we conclude that focal ischemic stroke increases vascular sensitivity to UDP-β-S by upregulating P2Y6 receptors on vascular smooth muscle cells while experimental SAH did not induce changes in vasoreactivity in spite of increased P2Y6 receptor expression.

Original languageEnglish
Article numbere15283
JournalPhysiological Reports
Volume10
Issue number8
Pages (from-to)e15283
ISSN2051-817X
DOIs
Publication statusPublished - Apr 2022

Bibliographical note

© 2022 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society.

    Research areas

  • Animals, Infarction, Middle Cerebral Artery, Ischemia, Rats, Receptors, Purinergic P2, Stroke, Uridine Diphosphate/metabolism, Vasoconstriction

ID: 77811314