CD34 immunoreactivity and interstitial cells of Cajal in the human and mouse gastrointestinal tract

J M Vanderwinden, J J Rumessen, M H De Laet, J J Vanderhaeghen, S N Schiffmann

    75 Citations (Scopus)


    Immunoreactivity for the tyrosine kinase receptor Kit (Kit-ir) is an established marker for the interstitial cells of Cajal (ICC) of the gut. Recently, the presence of CD34 immunoreactivity (CD34-ir) has been reported in Kit-ir ICC around the myenteric plexus in human small intestine. Conversely, we observed that CD34-ir labeled Kit-negative fibroblast-like cells, closely adjacent to, but distinct from, the Kit-ir ICC. The existence of cells expressing both CD34-ir and Kit-ir remains controversial. CD34-ir and Kit-ir were studied by high-resolution confocal microscopy on cryostat sections of human and murine gut as well as murine whole-mounts, using specific antibodies raised to human and murine CD34, respectively. CD34-ir labeled numerous cells in all parts of the gut, in man and in mouse. CD34-ir was consistently observed in Kit-negative cells, distinct from the closely adjacent Kit-ir ICC. Thin processes of both cell types intermingled extensively, often at the limit of resolution for light microscopy. CD34-ir was also observed in Kit-negative mesenchymal cells in the submucosa, in capillaries and in mesothelial cells. CD34-ir is not a marker for Kit-ir ICC in the human and murine gut. No CD34-ir, Kit-ir-expressing cells were encountered. Conversely, CD34-ir cells, closely adjacent to, but distinct from, Kit-ir ICC were consistently identified. The intimate relationship between these cells may offer an alternative explanation for reports of CD34 and Kit co-localization. The ontogeny and function of CD34-ir cells in the gut, as well as the origin of gastrointestinal stromal tumors, remain unclear.
    Original languageEnglish
    JournalCell and Tissue Research
    Issue number2
    Pages (from-to)145-53
    Number of pages9
    Publication statusPublished - Nov 2000


    • Animals
    • Antigens, CD34
    • Colon
    • Enteric Nervous System
    • Fluorescent Antibody Technique
    • Humans
    • Intestine, Small
    • Mice
    • Muscle, Smooth
    • Proto-Oncogene Proteins c-kit
    • Stomach


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