Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

CD34+ cells in human intestine are fibroblasts adjacent to, but distinct from, interstitial cells of Cajal

Research output: Contribution to journalJournal articleResearch

  1. Enzyme-free digital counting of endogenous circular RNA molecules in B-cell malignancies

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Expression of immunohistochemical markers for testicular carcinoma in situ by normal human fetal germ cells

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Light- and electron microscopical studies of interstitial cells of Cajal and muscle cells at the submucosal border of human colon

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. The association of celiac disease and allergic disease in a general adult population

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Medicinsk Kompendium: Tarmsygdomme

    Research output: Chapter in Book/Report/Conference proceedingBook chapterEducationpeer-review

  3. A low-gluten diet induces changes in the intestinal microbiome of healthy Danish adults

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. The distribution of HLA DQ2 and DQ8 haplotypes and their association with health indicators in a general Danish population

    Research output: Contribution to journalJournal articleResearchpeer-review

  • J M Vanderwinden
  • J J Rumessen
  • M H De Laet
  • J J Vanderhaeghen
  • S N Schiffmann
View graph of relations
Interstitial cells of Cajal (ICC) generate the pacemaker component of the gut and play important roles in the control of gut motility. The tyrosine kinase receptor Kit is an established marker for ICC. Recently, it has been reported that immunoreactivity for the sialomucin CD34 may be present on ICC in human intestine. Gastrointestinal stromal tumors express both Kit and CD34, suggesting that these tumors may derive from ICC. We characterized the distribution of CD34 immunoreactivity at the cellular level in the normal human gut, using double immunofluorescence immunohistochemistry and confocal microscopy. CD34 immunoreactivity identified previously unrecognized cells closely adjacent to, but distinct from, the Kit immunoreactive ICC. These CD34 immunoreactive cells expressed the fibroblast marker prolyl 4-hydroxylase-whereas ICC did not-and were also distinct from smooth muscle cells, glial cells, and macrophages. In the human gut, CD34 immunoreactivity is not expressed by ICC but by a population of fibroblasts, likely corresponding to the "fibroblast-like cells" described in previous ultrastructural studies. Our findings also challenge the hypothesis that stromal tumors originate from ICC.
Original languageEnglish
JournalLaboratory investigation; a journal of technical methods and pathology
Volume79
Issue number1
Pages (from-to)59-65
Number of pages7
ISSN0023-6837
Publication statusPublished - Jan 1999

    Research areas

  • Antigens, CD34, Cell Differentiation, Child, Child, Preschool, Fibroblasts, Humans, Immunohistochemistry, Infant, Intestines, Male, Stromal Cells

ID: 39690218