Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

CD1d knockout mice exhibit aggravated contact hypersensitivity responses due to reduced interleukin-10 production predominantly by regulatory B cells

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Carbonic anhydrases in human keratinocytes and their regulation by all-trans retinoic acid and 1α,25-dihydroxyvitamin D3

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. The role of skin barrier in occupational contact dermatitis

    Research output: Contribution to journalReviewResearchpeer-review

  1. Gene expression profiling in persons with multiple chemical sensitivity before and after a controlled n-butanol exposure session

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Effect of dietary gluten on dendritic cells and innate immune subsets in BALB/c and NOD mice

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Immune responses to hair dyes containing toluene-2,5-diamine

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Systemic immunogenicity of para-Phenylenediamine and Diphenylcyclopropenone: two potent contact allergy-inducing haptens

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Inverse Association between Rheumatoid Arthritis and Contact Allergy

    Research output: Contribution to journalJournal articleResearchpeer-review

  • Jonas Fjelbye
  • Julie C Antvorskov
  • Karsten Buschard
  • Shohreh Issazadeh-Navikas
  • Kåre Engkilde
View graph of relations

Conflicting observations have been reported concerning the role of CD1d-dependent natural killer T (NKT) cells in contact hypersensitivity (CHS), supporting either a disease-promoting or downregulatory function. We studied the role of NKT cells in CHS by comparing the immune response in CD1d knockout (CD1d KO) and wild-type (Wt) mice after contact allergen exposure. For induction of CHS, C57BL/6 CD1d KO mice (n = 6) and C57BL/6 Wt mice (n = 6) were sensitised with 1% (w/v) dinitrochlorobenzene (DNCB) or vehicle for three consecutive days and subsequently challenged with a single dose of 0.5% DNCB (w/v) on the ears fifteen days later. We demonstrate that CD1d KO mice, as compared with Wt littermates, have more pronounced infiltration of mononuclear cells in the skin (29.1% increase; P < 0.001), lower frequencies of interleukin-10(+) B cells (Bregs ) in the spleen (53.2% decrease; P < 0.05) and peritoneal cavity (80.8% decrease; P < 0.05) and increased production of interferon-γ (3-fold; P < 0.05) after DNCB sensitisation and challenge, which suggests an important regulatory and protective role of CD1d-dependent NKT cells in CHS in our model, at least in part via regulation of IL-10 producing Bregs .

Original languageEnglish
JournalExperimental Dermatology
Volume24
Issue number11
Pages (from-to)853-6
Number of pages4
ISSN0906-6705
DOIs
Publication statusPublished - Nov 2015

ID: 46179009