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Cardiomyocyte expression and cell-specific processing of procholecystokinin

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  1. Cardiac natriuretic peptides

    Research output: Contribution to journalReviewResearchpeer-review

  2. Catheter ablation and mortality, stroke and heart failure readmission with atrial fibrillation

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  3. Gut Mucosal Gene Expression and Metabolic Changes After Roux-en-Y Gastric Bypass Surgery

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  4. Limited diagnostic utility of Chromogranin A measurements in workup of neuroendocrine tumors

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Heart muscle cells produce peptide hormones such as natriuretic peptides. Developing hearts also express the gene for the classic intestinal hormone cholecystokinin (CCK) in amounts similar to those in the intestine and brain. However, cardiac expression of peptides other than natriuretic peptides has only been suggested using transcriptional measures or methods, with the post-translational phase of gene expression unaddressed. In this study, we examined the cardiac expression of the CCK gene in adult mammals and its expression at the protein level. Using quantitative PCR, a library of sequence-specific pro-CCK assays, peptide purification, and mass spectrometry, we demonstrate that the mammalian heart expresses pro-CCK in amounts comparable to natriuretic prohormones and processes it to a unique, triple-sulfated, and N-terminally truncated product distinct from intestinal and cerebral CCK peptides. Isoprenaline rapidly stimulated cardiac CCK gene expression in vitro and in vivo, which suggests that the cardiac-specific truncated pro-CCK may have pathophysiological relevance as a new marker of heart failure. The suggestion is confirmed by measurement of plasma from heart failure patients.

Original languageEnglish
JournalThe journal of biological chemistry
Issue number11
Pages (from-to)6837-43
Number of pages7
Publication statusPublished - 13 Mar 2015

    Research areas

  • Aged, Aged, 80 and over, Amino Acid Sequence, Animals, Cardiotonic Agents, Cell Line, Cholecystokinin, Female, Gene Expression, Heart Failure, Humans, Isoproterenol, Male, Middle Aged, Molecular Sequence Data, Myocytes, Cardiac, Prognosis, Protein Precursors, Rats, Swine

ID: 45860385