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Calcium-independent phospholipase A2 regulates retinal pigment epithelium proliferation and may be important in the pathogenesis of retinal diseases

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  1. COMPARATIVE EFFECTIVENESS OF PROTON BEAM VERSUS PHOTODYNAMIC THERAPY TO SPARE THE VISION IN CIRCUMSCRIBED CHOROIDAL HEMANGIOMA

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  2. Predicting Postoperative Vision for Macular Hole with Automated Image Analysis

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  3. Monocular and binocular end-points after epiretinal membrane surgery and their correlation to patient-reported outcomes

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  4. Simulation of advanced cataract surgery - validation of a newly developed test

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Calcium-independent phospholipase A2, group VIA (iPLA2-VIA) is involved in cell proliferation. This study aimed to evaluate the role of iPLA2-VIA in retinal pigment epithelium (RPE) cell proliferation and in retinal diseases involving RPE proliferation. A human RPE cell line (ARPE-19) was used to explore this role in vitro. Proliferating ARPE-19 cells had increased expression and activity of iPLA2-VIA. iPLA2-VIA was found in the nuclei of proliferating ARPE-19 cells, whereas in confluent ARPE-19 cells, with limited proliferation, iPLA2-VIA was primarily found in the cytosol. Inhibition of iPLA2-VIA decreased the rate of proliferation, whereas over expression of iPLA2-VIA increased the rate of proliferation. Using an experimental porcine model of RPE proliferation we demonstrated significant nuclear upregulation of iPLA2-VIA in proliferating RPE cells in vivo. We furthermore evaluated the expression of iPLA2-VIA in proliferative vitreoretinopathy (PVR). PVR membranes revealed nuclear expression of iPLA2-VIA in the RPE cells which had migrated and participated in the formation of the membranes. Overall, the present results point to an important role of iPLA2-VIA in the regulation of RPE proliferation suggesting that iPLA2-VIA may be considered as a possible pharmaceutical target in retinal diseases involving RPE proliferation and migration.
Original languageEnglish
JournalExperimental Eye Research
Volume89
Issue number3
Pages (from-to)383-91
Number of pages9
ISSN0014-4835
DOIs
Publication statusPublished - 1 Sep 2009

    Research areas

  • Alternative Splicing, Animals, Cell Nucleus, Cell Proliferation, Cells, Cultured, Endoplasmic Reticulum, Gene Silencing, Humans, Phospholipases A2, Calcium-Independent, RNA, Small Interfering, Retinal Pigment Epithelium, Sus scrofa, Vitreoretinopathy, Proliferative

ID: 30973254