Abstract
In order to identify cytosolic proteins involved in control of granule exocytosis in human neutrophils, subcellular fractions enriched in each of the 3 major granule subsets were incubated with cytosol from neutrophils in the presence or absence of Ca2+. After washing, proteins were eluted from the organelles by EGTA. Annexins I, II, IV and VI were found to bind to all organelles studied. In addition, a 28-kDa protein was found to bind exclusively to plasma membranes and secretory vesicles, the most readily exocytosed organelle of neutrophils. Ca(2+)-dependent association of cytosolic proteins to different granule subsets may control differential exocytosis of granules.
| Original language | English |
|---|---|
| Journal | FEBS Letters |
| Volume | 304 |
| Issue number | 2-3 |
| Pages (from-to) | 195-7 |
| Number of pages | 3 |
| ISSN | 0014-5793 |
| DOIs | |
| Publication status | Published - 15 Jun 1992 |
| Externally published | Yes |
Keywords
- Biological Transport, Active
- Calcium/pharmacology
- Cell Membrane/metabolism
- Cytoplasmic Granules/drug effects
- Cytosol/chemistry
- Exocytosis/drug effects
- Gelatinases
- Humans
- Neutrophils/chemistry
- Pepsin A/analysis
- Subcellular Fractions/enzymology
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