Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Breast Milk-Derived Extracellular Vesicles Enriched in Exosomes From Mothers With Type 1 Diabetes Contain Aberrant Levels of microRNAs

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Soluble Urokinase Plasminogen Activator Receptor (suPAR) as a Biomarker of Systemic Chronic Inflammation

    Research output: Contribution to journalReviewResearchpeer-review

  2. Mitochondrial-Linked De Novo Pyrimidine Biosynthesis Dictates Human T-Cell Proliferation but Not Expression of Effector Molecules

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Applicability of Small-Molecule Inhibitors in the Study of Peptidyl Arginine Deiminase 2 (PAD2) and PAD4

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Pregnancy outcomes in women with type 1 diabetes using insulin degludec

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Peptide Antibody Reactivity to Homologous Regions in Glutamate Decarboxylase Isoforms and Coxsackievirus B4 P2C

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. School performance in Danish children exposed to maternal type 1 diabetes in utero: A nationwide retrospective cohort study

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Increased liver fat associates with severe metabolic perturbations in low birth weight men

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

The breast milk plays a crucial role in shaping the initial intestinal microbiota and mucosal immunity of the infant. Interestingly, breastfeeding has proven to be protective against the early onset of immune-mediated diseases including type 1 diabetes. Studies have shown that exosomes from human breast milk are enriched in immune-modulating miRNAs suggesting that exosomal miRNAs (exomiRs) transferred to the infant could play a critical role in the development of the infant's immune system. We extracted exomiRs from breast milk of 52 lactating mothers (26 mothers with type 1 diabetes and 26 healthy mothers), to identify any differences in the exomiR content between the two groups. Small RNA-sequencing was performed to identify known and novel miRNAs in both groups. A total of 631 exomiRs were detected by small RNA sequencing including immune-related miRNAs such as hsa-let-7c, hsa-miR-21, hsa-miR-34a, hsa-miR-146b, and hsa-miR-200b. In addition, ~200 novel miRNAs were identified in both type 1 diabetes and control samples. Among the known miRNAs, nine exomiR's were found differentially expressed in mothers with type 1 diabetes compared to healthy mothers. The highly up-regulated miRNAs, hsa-miR-4497, and hsa-miR-3178, increased lipopolysaccharide-induced expression and secretion of tumor necrosis factor α (TNFα) in human monocytes. The up-regulated miRNA target genes were significantly enriched for longevity-regulating pathways and FoxO signaling. Our findings suggest a role of breast milk-derived exomiRs in modulating the infant's immune system.

Original languageEnglish
Article number2543
JournalFrontiers in Immunology
Volume10
Pages (from-to)1-10
Number of pages10
ISSN1664-3224
DOIs
Publication statusPublished - Oct 2019

    Research areas

  • Breast milk, ExomiRs, Exosomes, MiRNAs, Type 1 diabetes

ID: 58351358