Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Blood-brain barrier permeability measured using dynamic contrast-enhanced magnetic resonance imaging: a validation study

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Insulin-stimulated glucose uptake partly relies on p21-activated kinase (PAK)2, but not PAK1, in mouse skeletal muscle

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Heterogeneity in subcellular muscle glycogen utilisation during exercise impacts endurance capacity in men

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. The impact of loading, unloading, ageing and injury on the human tendon

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Human brain blood flow and metabolism during isocapnic hyperoxia: the role of reactive oxygen species

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Volume of hippocampal subregions and clinical improvement following electroconvulsive therapy in patients with depression

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Intradural artery dilation during experimentally induced migraine attacks

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Physiological responses of human skeletal muscle to acute blood flow restricted exercise assessed by multimodal MRI

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

KEY POINTS: The blood-brain barrier (BBB) is an important and dynamic structure which contributes to homeostasis in the central nervous system. BBB permeability changes occur in health and disease but measurement of BBB permeability in humans is not straightforward. Dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) can be used to model the movement of gadolinium contrast into the brain, expressed as the influx constant Ki . Here evidence is provided that Ki as measured by DCE-MRI behaves as expected for a marker of overall BBB leakage. These results support the use of DCE-MRI for in vivo studies of human BBB permeability in health and disease.

ABSTRACT: Blood-brain barrier (BBB) leakage can be measured using dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) as the influx constant Ki . To validate this method we compared measured Ki with biological expectations, namely (1) higher Ki in healthy individual grey matter (GM) versus white matter (WM), (2) GM/WM cerebral blood volume (CBV) ratio close to the histologically established GM/WM vascular density ratio, (3) higher Ki in visibly enhancing multiple sclerosis (MS) lesions versus MS normal appearing white matter (NAWM), and (4) higher Ki in MS NAWM versus healthy individual NAWM. We recruited 13 healthy individuals and 12 patients with MS and performed whole-brain 3D DCE-MRI at 3 T. Ki and CBV were calculated using Patlak modelling for manual regions of interest (ROI) and segmented tissue masks. Ki was higher in control GM versus WM (P = 0.001). CBV was higher in GM versus WM (P = 0.005, mean ratio 1.9). Ki was higher in visibly enhancing MS lesions versus MS NAWM (P = 0.002), and in MS NAWM versus controls (P = 0.014). Bland-Altman analysis showed no significant difference between ROI and segmentation methods (P = 0.638) and an intra-class correlation coefficient showed moderate single measure consistency (0.610). Ki behaves as expected for a compound marker of permeability and surface area. The GM/WM CBV ratio measured by this technique is in agreement with the literature. This adds evidence to the validity of Ki measured by DCE-MRI as a marker of overall BBB leakage.

Original languageEnglish
JournalThe Journal of physiology
Volume597
Issue number3
Pages (from-to)699-709
Number of pages11
ISSN0022-3751
DOIs
Publication statusPublished - Feb 2019

    Research areas

  • Blood-brain barrier, Cerebral blood flow, Magnetic Resonance Imaging

ID: 56535003