Baculovirus-expressed constructs induce immunoglobulin G that recognizes VAR2CSA on Plasmodium falciparum-infected erythrocytes

Lea Barfod, Morten A Nielsen, Louise Turner, Madeleine Dahlbäck, Anja T R Jensen, Lars Hviid, Thor G Theander, Ali Salanti

Abstract

We raised specific antisera against recombinant VAR2CSA domains produced in Escherichia coli and in insect cells. All were reactive in enzyme-linked immunosorbent assay, but only insect cell-derived constructs induced immunoglobulin G (IgG) that was reactive with native VAR2CSA on the surface of infected erythrocytes. Our data show that five of the six VAR2CSA Duffy-binding-like domains are surface exposed and that induction of surface-reactive VAR2CSA-specific IgG depends critically upon antigen conformation. These findings have implications for the development of vaccines against pregnancy-associated Plasmodium falciparum malaria.

Original languageEnglish
JournalInfection and Immunity
Volume74
Issue number7
Pages (from-to)4357-60
Number of pages4
ISSN0019-9567
DOIs
Publication statusPublished - Jul 2006
Externally publishedYes

Keywords

  • Animals
  • Antigens, Protozoan/immunology
  • Baculoviridae/genetics
  • Erythrocytes/immunology
  • Female
  • Genetic Vectors
  • Humans
  • Immune Sera/metabolism
  • Immunoglobulin G/biosynthesis
  • Mice
  • Plasmodium falciparum/immunology
  • Rabbits
  • Recombinant Proteins/immunology

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