Harvard
Zoran, T, Sartori, B, Sappl, L, Aigner, M, Sánchez-Reus, F, Rezusta, A, Chowdhary, A, Taj-Aldeen, SJ
, Arendrup, MC, Oliveri, S, Kontoyiannis, DP, Alastruey-Izquierdo, A, Lagrou, K, Cascio, GL, Meis, JF, Buzina, W, Farina, C, Drogari-Apiranthitou, M, Grancini, A, Tortorano, AM, Willinger, B, Hamprecht, A, Johnson, E, Klingspor, L, Arsic-Arsenijevic, V, Cornely, OA, Meletiadis, J, Prammer, W, Tullio, V, Vehreschild, J-J, Trovato, L, Lewis, RE, Segal, E, Rath, P-M, Hamal, P, Rodriguez-Iglesias, M, Roilides, E, Arikan-Akdagli, S, Chakrabarti, A, Colombo, AL, Fernández, MS, Martin-Gomez, MT, Badali, H, Petrikkos, G, Klimko, N, Heimann, SM, Uzun, O, Roudbary, M, de la Fuente, S & Houbraken, J 2018, '
Azole-Resistance in Aspergillus terreus and Related Species: An Emerging Problem or a Rare Phenomenon?',
Frontiers in Microbiology, vol. 9, pp. 516.
https://doi.org/10.3389/fmicb.2018.00516APA
Zoran, T., Sartori, B., Sappl, L., Aigner, M., Sánchez-Reus, F., Rezusta, A., Chowdhary, A., Taj-Aldeen, S. J.
, Arendrup, M. C., Oliveri, S., Kontoyiannis, D. P., Alastruey-Izquierdo, A., Lagrou, K., Cascio, G. L., Meis, J. F., Buzina, W., Farina, C., Drogari-Apiranthitou, M., Grancini, A., ... Houbraken, J. (2018).
Azole-Resistance in Aspergillus terreus and Related Species: An Emerging Problem or a Rare Phenomenon? Frontiers in Microbiology,
9, 516.
https://doi.org/10.3389/fmicb.2018.00516CBE
Zoran T, Sartori B, Sappl L, Aigner M, Sánchez-Reus F, Rezusta A, Chowdhary A, Taj-Aldeen SJ
, Arendrup MC, Oliveri S, Kontoyiannis DP, Alastruey-Izquierdo A, Lagrou K, Cascio GL, Meis JF, Buzina W, Farina C, Drogari-Apiranthitou M, Grancini A, Tortorano AM, Willinger B, Hamprecht A, Johnson E, Klingspor L, Arsic-Arsenijevic V, Cornely OA, Meletiadis J, Prammer W, Tullio V, Vehreschild J-J, Trovato L, Lewis RE, Segal E, Rath P-M, Hamal P, Rodriguez-Iglesias M, Roilides E, Arikan-Akdagli S, Chakrabarti A, Colombo AL, Fernández MS, Martin-Gomez MT, Badali H, Petrikkos G, Klimko N, Heimann SM, Uzun O, Roudbary M, de la Fuente S, Houbraken J. 2018.
Azole-Resistance in Aspergillus terreus and Related Species: An Emerging Problem or a Rare Phenomenon?.
Frontiers in Microbiology. 9:516.
https://doi.org/10.3389/fmicb.2018.00516MLA
Vancouver
Author
Zoran, Tamara ; Sartori, Bettina ; Sappl, Laura ; Aigner, Maria ; Sánchez-Reus, Ferran ; Rezusta, Antonio ; Chowdhary, Anuradha ; Taj-Aldeen, Saad J
; Arendrup, Maiken C ; Oliveri, Salvatore ; Kontoyiannis, Dimitrios P ; Alastruey-Izquierdo, Ana ; Lagrou, Katrien ; Cascio, Giuliana Lo ; Meis, Jacques F ; Buzina, Walter ; Farina, Claudio ; Drogari-Apiranthitou, Miranda ; Grancini, Anna ; Tortorano, Anna M ; Willinger, Birgit ; Hamprecht, Axel ; Johnson, Elizabeth ; Klingspor, Lena ; Arsic-Arsenijevic, Valentina ; Cornely, Oliver A ; Meletiadis, Joseph ; Prammer, Wolfgang ; Tullio, Vivian ; Vehreschild, Jörg-Janne ; Trovato, Laura ; Lewis, Russell E ; Segal, Esther ; Rath, Peter-Michael ; Hamal, Petr ; Rodriguez-Iglesias, Manuel ; Roilides, Emmanuel ; Arikan-Akdagli, Sevtap ; Chakrabarti, Arunaloke ; Colombo, Arnaldo L ; Fernández, Mariana S ; Martin-Gomez, M Teresa ; Badali, Hamid ; Petrikkos, Georgios ; Klimko, Nikolai ; Heimann, Sebastian M ; Uzun, Omrum ; Roudbary, Maryam ; de la Fuente, Sonia ; Houbraken, Jos. /
Azole-Resistance in Aspergillus terreus and Related Species : An Emerging Problem or a Rare Phenomenon?. In:
Frontiers in Microbiology. 2018 ; Vol. 9. pp. 516.
Bibtex
@article{42d271b29b624a1c8f5371ff48d5fa50,
title = "Azole-Resistance in Aspergillus terreus and Related Species: An Emerging Problem or a Rare Phenomenon?",
abstract = "Objectives: Invasive mold infections associated with Aspergillus species are a significant cause of mortality in immunocompromised patients. The most frequently occurring aetiological pathogens are members of the Aspergillus section Fumigati followed by members of the section Terrei. The frequency of Aspergillus terreus and related (cryptic) species in clinical specimens, as well as the percentage of azole-resistant strains remains to be studied. Methods: A global set (n = 498) of A. terreus and phenotypically related isolates was molecularly identified (beta-tubulin), tested for antifungal susceptibility against posaconazole, voriconazole, and itraconazole, and resistant phenotypes were correlated with point mutations in the cyp51A gene. Results: The majority of isolates was identified as A. terreus (86.8%), followed by A. citrinoterreus (8.4%), A. hortai (2.6%), A. alabamensis (1.6%), A. neoafricanus (0.2%), and A. floccosus (0.2%). One isolate failed to match a known Aspergillus sp., but was found most closely related to A. alabamensis. According to EUCAST clinical breakpoints azole resistance was detected in 5.4% of all tested isolates, 6.2% of A. terreus sensu stricto (s.s.) were posaconazole-resistant. Posaconazole resistance differed geographically and ranged from 0% in the Czech Republic, Greece, and Turkey to 13.7% in Germany. In contrast, azole resistance among cryptic species was rare 2 out of 66 isolates and was observed only in one A. citrinoterreus and one A. alabamensis isolate. The most affected amino acid position of the Cyp51A gene correlating with the posaconazole resistant phenotype was M217, which was found in the variation M217T and M217V. Conclusions:Aspergillus terreus was most prevalent, followed by A. citrinoterreus. Posaconazole was the most potent drug against A. terreus, but 5.4% of A. terreus sensu stricto showed resistance against this azole. In Austria, Germany, and the United Kingdom posaconazole-resistance in all A. terreus isolates was higher than 10%, resistance against voriconazole was rare and absent for itraconazole.",
author = "Tamara Zoran and Bettina Sartori and Laura Sappl and Maria Aigner and Ferran S{\'a}nchez-Reus and Antonio Rezusta and Anuradha Chowdhary and Taj-Aldeen, {Saad J} and Arendrup, {Maiken C} and Salvatore Oliveri and Kontoyiannis, {Dimitrios P} and Ana Alastruey-Izquierdo and Katrien Lagrou and Cascio, {Giuliana Lo} and Meis, {Jacques F} and Walter Buzina and Claudio Farina and Miranda Drogari-Apiranthitou and Anna Grancini and Tortorano, {Anna M} and Birgit Willinger and Axel Hamprecht and Elizabeth Johnson and Lena Klingspor and Valentina Arsic-Arsenijevic and Cornely, {Oliver A} and Joseph Meletiadis and Wolfgang Prammer and Vivian Tullio and J{\"o}rg-Janne Vehreschild and Laura Trovato and Lewis, {Russell E} and Esther Segal and Peter-Michael Rath and Petr Hamal and Manuel Rodriguez-Iglesias and Emmanuel Roilides and Sevtap Arikan-Akdagli and Arunaloke Chakrabarti and Colombo, {Arnaldo L} and Fern{\'a}ndez, {Mariana S} and Martin-Gomez, {M Teresa} and Hamid Badali and Georgios Petrikkos and Nikolai Klimko and Heimann, {Sebastian M} and Omrum Uzun and Maryam Roudbary and {de la Fuente}, Sonia and Jos Houbraken",
year = "2018",
doi = "10.3389/fmicb.2018.00516",
language = "English",
volume = "9",
pages = "516",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Research Foundation",
}
RIS
TY - JOUR
T1 - Azole-Resistance in Aspergillus terreus and Related Species
T2 - An Emerging Problem or a Rare Phenomenon?
AU - Zoran, Tamara
AU - Sartori, Bettina
AU - Sappl, Laura
AU - Aigner, Maria
AU - Sánchez-Reus, Ferran
AU - Rezusta, Antonio
AU - Chowdhary, Anuradha
AU - Taj-Aldeen, Saad J
AU - Arendrup, Maiken C
AU - Oliveri, Salvatore
AU - Kontoyiannis, Dimitrios P
AU - Alastruey-Izquierdo, Ana
AU - Lagrou, Katrien
AU - Cascio, Giuliana Lo
AU - Meis, Jacques F
AU - Buzina, Walter
AU - Farina, Claudio
AU - Drogari-Apiranthitou, Miranda
AU - Grancini, Anna
AU - Tortorano, Anna M
AU - Willinger, Birgit
AU - Hamprecht, Axel
AU - Johnson, Elizabeth
AU - Klingspor, Lena
AU - Arsic-Arsenijevic, Valentina
AU - Cornely, Oliver A
AU - Meletiadis, Joseph
AU - Prammer, Wolfgang
AU - Tullio, Vivian
AU - Vehreschild, Jörg-Janne
AU - Trovato, Laura
AU - Lewis, Russell E
AU - Segal, Esther
AU - Rath, Peter-Michael
AU - Hamal, Petr
AU - Rodriguez-Iglesias, Manuel
AU - Roilides, Emmanuel
AU - Arikan-Akdagli, Sevtap
AU - Chakrabarti, Arunaloke
AU - Colombo, Arnaldo L
AU - Fernández, Mariana S
AU - Martin-Gomez, M Teresa
AU - Badali, Hamid
AU - Petrikkos, Georgios
AU - Klimko, Nikolai
AU - Heimann, Sebastian M
AU - Uzun, Omrum
AU - Roudbary, Maryam
AU - de la Fuente, Sonia
AU - Houbraken, Jos
PY - 2018
Y1 - 2018
N2 - Objectives: Invasive mold infections associated with Aspergillus species are a significant cause of mortality in immunocompromised patients. The most frequently occurring aetiological pathogens are members of the Aspergillus section Fumigati followed by members of the section Terrei. The frequency of Aspergillus terreus and related (cryptic) species in clinical specimens, as well as the percentage of azole-resistant strains remains to be studied. Methods: A global set (n = 498) of A. terreus and phenotypically related isolates was molecularly identified (beta-tubulin), tested for antifungal susceptibility against posaconazole, voriconazole, and itraconazole, and resistant phenotypes were correlated with point mutations in the cyp51A gene. Results: The majority of isolates was identified as A. terreus (86.8%), followed by A. citrinoterreus (8.4%), A. hortai (2.6%), A. alabamensis (1.6%), A. neoafricanus (0.2%), and A. floccosus (0.2%). One isolate failed to match a known Aspergillus sp., but was found most closely related to A. alabamensis. According to EUCAST clinical breakpoints azole resistance was detected in 5.4% of all tested isolates, 6.2% of A. terreus sensu stricto (s.s.) were posaconazole-resistant. Posaconazole resistance differed geographically and ranged from 0% in the Czech Republic, Greece, and Turkey to 13.7% in Germany. In contrast, azole resistance among cryptic species was rare 2 out of 66 isolates and was observed only in one A. citrinoterreus and one A. alabamensis isolate. The most affected amino acid position of the Cyp51A gene correlating with the posaconazole resistant phenotype was M217, which was found in the variation M217T and M217V. Conclusions:Aspergillus terreus was most prevalent, followed by A. citrinoterreus. Posaconazole was the most potent drug against A. terreus, but 5.4% of A. terreus sensu stricto showed resistance against this azole. In Austria, Germany, and the United Kingdom posaconazole-resistance in all A. terreus isolates was higher than 10%, resistance against voriconazole was rare and absent for itraconazole.
AB - Objectives: Invasive mold infections associated with Aspergillus species are a significant cause of mortality in immunocompromised patients. The most frequently occurring aetiological pathogens are members of the Aspergillus section Fumigati followed by members of the section Terrei. The frequency of Aspergillus terreus and related (cryptic) species in clinical specimens, as well as the percentage of azole-resistant strains remains to be studied. Methods: A global set (n = 498) of A. terreus and phenotypically related isolates was molecularly identified (beta-tubulin), tested for antifungal susceptibility against posaconazole, voriconazole, and itraconazole, and resistant phenotypes were correlated with point mutations in the cyp51A gene. Results: The majority of isolates was identified as A. terreus (86.8%), followed by A. citrinoterreus (8.4%), A. hortai (2.6%), A. alabamensis (1.6%), A. neoafricanus (0.2%), and A. floccosus (0.2%). One isolate failed to match a known Aspergillus sp., but was found most closely related to A. alabamensis. According to EUCAST clinical breakpoints azole resistance was detected in 5.4% of all tested isolates, 6.2% of A. terreus sensu stricto (s.s.) were posaconazole-resistant. Posaconazole resistance differed geographically and ranged from 0% in the Czech Republic, Greece, and Turkey to 13.7% in Germany. In contrast, azole resistance among cryptic species was rare 2 out of 66 isolates and was observed only in one A. citrinoterreus and one A. alabamensis isolate. The most affected amino acid position of the Cyp51A gene correlating with the posaconazole resistant phenotype was M217, which was found in the variation M217T and M217V. Conclusions:Aspergillus terreus was most prevalent, followed by A. citrinoterreus. Posaconazole was the most potent drug against A. terreus, but 5.4% of A. terreus sensu stricto showed resistance against this azole. In Austria, Germany, and the United Kingdom posaconazole-resistance in all A. terreus isolates was higher than 10%, resistance against voriconazole was rare and absent for itraconazole.
U2 - 10.3389/fmicb.2018.00516
DO - 10.3389/fmicb.2018.00516
M3 - Journal article
C2 - 29643840
VL - 9
SP - 516
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
SN - 1664-302X
ER -
