Autofluorescence in samples obtained from chronic biofilm infections - "all that glitters is not gold"

Steffen Eickhardt, Kasper Nørskov Kragh, Stine Schrøder, Steen Seier Poulsen, Henrik Sillesen, Michael Givskov, Niels Høiby, Thomas Bjarnsholt, Morten Alhede

13 Citations (Scopus)

Abstract

When looking at tissue sections of ex vivo samples, autofluorescence can be a major cause of artifacts and misinterpretations. We here reiterate evidence that autofluorescing granules, often hemosiderin but also ceroid or mucinogen granules, are severe obstacles when imaging and diagnosing biofilm infections through fluorescent imaging techniques. We used confocal laser scanning microscopy with spectral analysis for autofluorescence detection as well as standard histological stains in order to identify the culprit and show that these granules might very well be mistaken for bacterial biofilms. Furthermore, we hypothesize that the increased amount of autofluorescing granules may be a consequence of prolonged inflammation as a consequence of chronic biofilm infections.

Original languageEnglish
JournalPathogens and Disease
Volume73
Issue number4
Pages (from-to)pii
Number of pages4
DOIs
Publication statusPublished - Jun 2015

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