Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Anti-Campylobacter activity of resveratrol and an extract from waste Pinot noir grape skins and seeds, and resistance of Camp. jejuni planktonic and biofilm cells, mediated via the CmeABC efflux pump

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Biofilm and motility in response to environmental and host-related signals in Gram negative opportunistic pathogens

    Research output: Contribution to journalReviewResearchpeer-review

  2. New filtration system for efficient recovery of waterborne Cryptosporidium oocysts and Giardia cysts

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Describing the fecal metabolome in cryogenically collected samples from healthy participants

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Circulating metabolites in progression to islet autoimmunity and type 1 diabetes

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Impact of binge drinking on hepatic lipid metabolism: lipidome analysis of liver vein and peripheral blood during acute alcohol intoxication

    Research output: Contribution to journalConference abstract in journalResearchpeer-review

  4. Polyols and Branched Chained Amino Acids Are Associated with Present and Future Renal Impairment in Type 1 Diabetes

    Research output: Contribution to journalConference abstract in journalResearchpeer-review

  • A. Klančnik
  • M. Šikić Pogačar
  • K. Trošt
  • M. Tušek Žnidarič
  • B. Mozetič Vodopivec
  • S. Smole Možina
View graph of relations

Aims: To define anti-Campylobacter jejuni activity of an extract from waste skins and seeds of Pinot noir grapes (GSS), resveratrol and possible resistance mechanisms, and the influence of these on Camp. jejuni morphology. Methods and Results: Using gene-specific knock-out Camp. jejuni mutants and an efflux pump inhibitor, we showed CmeABC as the most active efflux pump for extrusion across the outer membrane of GSS extract and resveratrol. Using polystyrene surface and pig small intestine epithelial (PSI) and human foetal small intestine (H4) cell lines, GSS extract shows an efficient inhibition of adhesion of Camp. jejuni to these abiotic and biotic surfaces. Conclusions: Low doses of GSS extract can inhibit Camp. jejuni adhesion to polystyrene surfaces and to PSI and H4 cells, and can thus modulate Camp. jejuni invasion and intracellular survival. Significance and Impact of the Study: An understanding of the activities of GSS extract and resveratrol as bacterial growth inhibitors and the specific mechanisms of cell accumulation is crucial for our understanding of Camp. jejuni resistance. GSS extract inhibition of Camp. jejuni adhesion to abiotic and biotic surfaces provides a further step towards the application of new innovative strategies to control Campylobacter contamination and infection via the food chain.

Original languageEnglish
JournalJournal of Applied Microbiology
Volume122
Issue number1
Pages (from-to)65-77
Number of pages13
ISSN1364-5072
DOIs
Publication statusPublished - 1 Jan 2017
Externally publishedYes

    Research areas

  • abiotic/biotic surface, antiadhesion, bioactive by-products, Campylobacter jejuni, efflux pump

ID: 52586113