Antenatal bestemmelse af føtal RhD-blodtype baseret på føtalt DNA i plasma fra den RhD-negative mor--sekundaerpublikation

Frederik Banch Clausen; Grethe Risum Krog; Klaus Rieneck; Leif Kofoed Nielsen; Rasmus Lundquist; Kirsten Finning; Ebbe Dickmeiss; Morten Hedegaard; Morten Hanefeld Dziegiel

Antenatal bestemmelse af føtal RhD-blodtype baseret på føtalt DNA i plasma fra den RhD-negative mor--sekundaerpublikation

Translated title of the contribution: Antenatal determination of fetal RhD-blood type based on fetal DNA in plasma from the RhD-negative mother--secondary publication

Frederik Banch Clausen, Grethe Risum Krog, Klaus Rieneck, Leif Kofoed Nielsen, Rasmus Lundquist, Kirsten Finning, Ebbe Dickmeiss, Morten Hedegaard, Morten Hanefeld Dziegiel

2 Citations (Scopus)

Abstract

We present a reliable test for prenatal prediction of fetal RhD type using maternal plasma from RhD-women. This test is needed for a future antenatal RH prophylaxis. A new real time PCR based assay targeting RHD exon 7 and a published assay for RHD exon 10 were used to determine the fetal RHD status in DNA extracted from plasma from 56 pregnant women in 15th-36th week of gestation. Prediction of fetal RhD type was compared with the phenotype determined after birth, and showed 100% concordance. This setup will be of value in antenatal RH prophylaxis and in the management of immunised women.

Translated title of the contribution	Antenatal determination of fetal RhD-blood type based on fetal DNA in plasma from the RhD-negative mother--secondary publication
Original language	Danish
Journal	Ugeskrift for Laeger
Volume	168
Issue number	26-32
Pages (from-to)	2568-70
Number of pages	3
ISSN	0041-5782
Publication status	Published - 26 Jun 2006

Cite this

@article{7e8121d16791412a975edf3585b97e16,

title = "Antenatal bestemmelse af f{\o}tal RhD-blodtype baseret p{\aa} f{\o}talt DNA i plasma fra den RhD-negative mor--sekundaerpublikation",

abstract = "We present a reliable test for prenatal prediction of fetal RhD type using maternal plasma from RhD-women. This test is needed for a future antenatal RH prophylaxis. A new real time PCR based assay targeting RHD exon 7 and a published assay for RHD exon 10 were used to determine the fetal RHD status in DNA extracted from plasma from 56 pregnant women in 15th-36th week of gestation. Prediction of fetal RhD type was compared with the phenotype determined after birth, and showed 100\% concordance. This setup will be of value in antenatal RH prophylaxis and in the management of immunised women.",

keywords = "DNA, DNA Probes, Exons, Female, Fetal Diseases, Genotype, Humans, Polymerase Chain Reaction, Pregnancy, Prenatal Diagnosis, Rh-Hr Blood-Group System, Sensitivity and Specificity",

author = "Clausen, \{Frederik Banch\} and Krog, \{Grethe Risum\} and Klaus Rieneck and Nielsen, \{Leif Kofoed\} and Rasmus Lundquist and Kirsten Finning and Ebbe Dickmeiss and Morten Hedegaard and Dziegiel, \{Morten Hanefeld\}",

year = "2006",

month = jun,

day = "26",

language = "Dansk",

volume = "168",

pages = "2568--70",

journal = "Ugeskrift for Laeger",

issn = "0041-5782",

publisher = "Almindelige Danske L{\ae}geforening",

number = "26-32",

}

TY - JOUR

T1 - Antenatal bestemmelse af føtal RhD-blodtype baseret på føtalt DNA i plasma fra den RhD-negative mor--sekundaerpublikation

AU - Clausen, Frederik Banch

AU - Krog, Grethe Risum

AU - Rieneck, Klaus

AU - Nielsen, Leif Kofoed

AU - Lundquist, Rasmus

AU - Finning, Kirsten

AU - Dickmeiss, Ebbe

AU - Hedegaard, Morten

AU - Dziegiel, Morten Hanefeld

PY - 2006/6/26

Y1 - 2006/6/26

N2 - We present a reliable test for prenatal prediction of fetal RhD type using maternal plasma from RhD-women. This test is needed for a future antenatal RH prophylaxis. A new real time PCR based assay targeting RHD exon 7 and a published assay for RHD exon 10 were used to determine the fetal RHD status in DNA extracted from plasma from 56 pregnant women in 15th-36th week of gestation. Prediction of fetal RhD type was compared with the phenotype determined after birth, and showed 100% concordance. This setup will be of value in antenatal RH prophylaxis and in the management of immunised women.

AB - We present a reliable test for prenatal prediction of fetal RhD type using maternal plasma from RhD-women. This test is needed for a future antenatal RH prophylaxis. A new real time PCR based assay targeting RHD exon 7 and a published assay for RHD exon 10 were used to determine the fetal RHD status in DNA extracted from plasma from 56 pregnant women in 15th-36th week of gestation. Prediction of fetal RhD type was compared with the phenotype determined after birth, and showed 100% concordance. This setup will be of value in antenatal RH prophylaxis and in the management of immunised women.

KW - DNA

KW - DNA Probes

KW - Exons

KW - Female

KW - Fetal Diseases

KW - Genotype

KW - Humans

KW - Polymerase Chain Reaction

KW - Pregnancy

KW - Prenatal Diagnosis

KW - Rh-Hr Blood-Group System

KW - Sensitivity and Specificity

UR - https://www.scopus.com/pages/publications/33746581408

M3 - Tidsskriftartikel

C2 - 16824414

SN - 0041-5782

VL - 168

SP - 2568

EP - 2570

JO - Ugeskrift for Laeger

JF - Ugeskrift for Laeger

IS - 26-32

ER -

Antenatal bestemmelse af føtal RhD-blodtype baseret på føtalt DNA i plasma fra den RhD-negative mor--sekundaerpublikation

Abstract

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