Antagonism of the interleukin 4 receptor α promotes TH 1-signalling among T cells from patients with atopic dermatitis after stimulation

11 Citations (Scopus)

Abstract

Atopic dermatitis (AD) is a chronic and relapsing inflammatory skin disease. Molecular characterization of AD shows an underlying inflammation with tissue infiltration of T helper (TH ) 2 cells and increased IL-4 and IL-13. The multifaceted roles of IL-4 and IL-13 in allergic disease development make IL-4Rα an attractive target for treatment strategies, and a neutralizing monoclonal antibody which antagonizes the effects of both IL-4 and IL-13 by blocking the interaction site found in the IL-4 receptor subunit α (IL-4Rα) has been successfully used to treat patients with moderate-to-severe AD. To elucidate the effects of IL-4Rα blockade on the cellular level, we used flow cytometry to examine cytokine production after antigen stimulation in human T cells from patients with AD (n = 12) and healthy controls (n = 6). The cells were stimulated with and without a neutralizing monoclonal antibody against IL-4Rα. Our results indicate that blocking IL-4Rα prohibits IL-4 signalling and IL-13 signalling and thereby TH 2 differentiation followed by an upregulation of interferon-γ-producing cells.

Original languageEnglish
Article numbere12835
JournalScandinavian Journal of Immunology
Volume91
Issue number1
Pages (from-to)e12835
ISSN0300-9475
DOIs
Publication statusPublished - Jan 2020

Keywords

  • Adult
  • Biomarkers
  • Cytokines/metabolism
  • Dermatitis, Atopic/immunology
  • Female
  • Humans
  • Immunoglobulin E/blood
  • Interleukin-4 Receptor alpha Subunit/antagonists & inhibitors
  • Leukocytes, Mononuclear/immunology
  • Male
  • Middle Aged
  • Signal Transduction
  • T-Lymphocyte Subsets/immunology
  • Th1 Cells/immunology
  • Young Adult
  • atopic dermatitis
  • dupilumab
  • CD4
  • IL4Rα
  • T helper cells
  • IL4R alpha

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