Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Analysis of gene expression profiles of microdissected cell populations indicates that testicular carcinoma in situ is an arrested gonocyte

Research output: Contribution to journalJournal articleResearch

  1. 'Snail factors in testicular germ cell tumours and their regulation by the BMP4 signalling pathway'

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Activin A determines steroid levels and composition in the fetal testis

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Distinguishing between hidden testes and anorchia: The role of endocrine evaluation in infancy and childhood

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Luteinizing Hormone Receptor Is Expressed in Testicular Germ Cell Tumors: Possible Implications for Tumor Growth and Prognosis

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations
Testicular germ cell cancers in young adult men derive from a precursor lesion called carcinoma in situ (CIS) of the testis. CIS cells were suggested to arise from primordial germ cells or gonocytes. However, direct studies on purified samples of CIS cells are lacking. To overcome this problem, we performed laser microdissection of CIS cells. Highly enriched cell populations were obtained and subjected to gene expression analysis. The expression profile of CIS cells was compared with microdissected gonocytes, oogonia, and cultured embryonic stem cells with and without genomic aberrations. Three samples of each tissue type were used for the analyses. Unique expression patterns for these developmentally very related cell types revealed that CIS cells were very similar to gonocytes because only five genes distinguished these two cell types. We did not find indications that CIS was derived from a meiotic cell, and the similarity to embryonic stem cells was modest compared with gonocytes. Thus, we provide new evidence that the molecular phenotype of CIS cells is similar to that of gonocytes. Our data are in line with the idea that CIS cells may be gonocytes that survived in the postnatal testis. We speculate that disturbed development of somatic cells in the fetal testis may play a role in allowing undifferentiated cells to survive in the postnatal testes. The further development of CIS into invasive germ cell tumors may depend on signals from their postpubertal niche of somatic cells, including hormones and growth factors from Leydig and Sertoli cells.
Original languageEnglish
Book seriesCurrent Cancer Research
Volume69
Issue number12
Pages (from-to)5241-50
Number of pages10
ISSN0940-0745
DOIs
Publication statusPublished - 15 Jun 2009

ID: 31058777