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Analysis by Flow Cytometry of α2-Macroglobulin and Nonimmune IgM-Binding to Plasmodium falciparum-Infected Erythrocytes

Research output: Contribution to journalJournal articlepeer-review

  1. Analysis of var Gene Transcript Patterns by Quantitative Real-Time PCR

    Research output: Contribution to journalJournal articlepeer-review

  2. Isolation and Characterization of Human Brown Adipocytes

    Research output: Contribution to journalJournal articlepeer-review

  3. Production of PfEMP1-Specific Human Monoclonal Antibodies from Naturally Immune Individuals

    Research output: Contribution to journalJournal articlepeer-review

  4. Production of PfEMP1-Specific Mouse Monoclonal Antibodies

    Research output: Contribution to journalJournal articlepeer-review

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Several members of the Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family can bind human serum proteins such as IgM and α2-macroglobulin (α2M). This binding seems to play a role in pathogenesis and immune evasion by improving the avidity of PfEMP1-mediated binding to erythrocyte receptors and/or by masking antibody epitopes in PfEMP1. In this protocol, we describe a flow cytometry-based protocol to evaluate IgM- and α2M-binding to intact and unfixed mature-stage IEs. The method can be used for laboratory clones and field isolates.

Original languageEnglish
JournalMethods in Molecular Biology
Volume2470
Pages (from-to)435-444
Number of pages10
ISSN1064-3745
DOIs
Publication statusPublished - 2022

Bibliographical note

© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

ID: 79740707