TY - JOUR
T1 - An exploratory study of microbial diversity in sinus infections of cystic fibrosis patients by molecular methods
AU - Rudkjøbing, Vibeke Børsholt
AU - Aanaes, Kasper
AU - Wolff, Tine Yding
AU - von Buchwald, Christian
AU - Johansen, Helle Krogh
AU - Thomsen, Trine Rolighed
N1 - Copyright © 2014 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.
PY - 2014/12
Y1 - 2014/12
N2 - BACKGROUND: For the first time microorganisms in CF sinuses are investigated by molecular methods in response to an absence of anaerobes in CF sinus samples during a two-year period at the Copenhagen CF center.METHODS: Endoscopic sinus surgery was performed in 19 CF patients. DNA from intact bacterial cells was investigated by 16S rRNA gene analysis and quantitative PCR. Results were compared to culture-dependent routine diagnosis.RESULTS: Molecular methods showed a large microbial diversity, which included undetected anaerobes that may play a pathogenic role. Importantly, the culture methods did not always detect known CF pathogens. Quantitative PCR showed generally a higher abundance of classic CF pathogens e.g. Pseudomonas aeruginosa and Staphylococcus aureus compared with the anaerobe Propionibacterium acnes.CONCLUSIONS: The results indicate that the culture methods in some cases may not be suitable as stand-alone method for this patient group, as diversity may be underestimated and important species undetected.
AB - BACKGROUND: For the first time microorganisms in CF sinuses are investigated by molecular methods in response to an absence of anaerobes in CF sinus samples during a two-year period at the Copenhagen CF center.METHODS: Endoscopic sinus surgery was performed in 19 CF patients. DNA from intact bacterial cells was investigated by 16S rRNA gene analysis and quantitative PCR. Results were compared to culture-dependent routine diagnosis.RESULTS: Molecular methods showed a large microbial diversity, which included undetected anaerobes that may play a pathogenic role. Importantly, the culture methods did not always detect known CF pathogens. Quantitative PCR showed generally a higher abundance of classic CF pathogens e.g. Pseudomonas aeruginosa and Staphylococcus aureus compared with the anaerobe Propionibacterium acnes.CONCLUSIONS: The results indicate that the culture methods in some cases may not be suitable as stand-alone method for this patient group, as diversity may be underestimated and important species undetected.
U2 - 10.1016/j.jcf.2014.02.008
DO - 10.1016/j.jcf.2014.02.008
M3 - Journal article
C2 - 24636809
SN - 1569-1993
VL - 13
SP - 645
EP - 652
JO - Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society
JF - Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society
IS - 6
ER -