Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

An exploration of the methods to determine the protein-specific synthesis and breakdown rates in vivo in humans

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Hypovolemia and reduced hemoglobin mass in patients with heart failure and preserved ejection fraction

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Acute intramyocardial lipid accumulation in rats does not slow cardiac conduction per se

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Age-dependent impairment of the erythropoietin response to reduced central venous pressure in HFpEF patients

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Postprandial Nutrient Handling and Gastrointestinal Hormone Secretion After Roux-en-Y Gastric Bypass vs Sleeve Gastrectomy

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Sequence variants in muscle tissue-related genes may determine the severity of muscle contractures in cerebral palsy

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Nasal insulin administration does not affect hepatic glucose production at systemic fasting insulin levels

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Training state and skeletal muscle autophagy in response to 36 h of fasting

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

The present study explores the methods to determine human in vivo protein-specific myofibrillar and collagenous connective tissue protein fractional synthesis and breakdown rates. We found that in human myofibrillar proteins, the protein-bound tracer disappearance method to determine the protein fractional breakdown rate (FBR) (via 2 H2 O ingestion, endogenous labeling of 2 H-alanine that is incorporated into proteins, and FBR quantified by its disappearance from these proteins) has a comparable intrasubject reproducibility (range: 0.09-53.5%) as the established direct-essential amino acid, here L-ring-13 C6 -phenylalanine, incorporation method to determine the muscle protein fractional synthesis rate (FSR) (range: 2.8-56.2%). Further, the determination of the protein breakdown in a protein structure with complex post-translational processing and maturation, exemplified by human tendon tissue, was not achieved in this experimentation, but more investigation is encouraged to reveal the possibility. Finally, we found that muscle protein FBR measured with an essential amino acid tracer prelabeling is inappropriate presumably because of significant and prolonged intracellular recycling, which also may become a significant limitation for determination of the myofibrillar FSR when repeated infusion trials are completed in the same participants.

Original languageEnglish
Article numbere14143
JournalPhysiological Reports
Volume7
Issue number17
ISSN2051-817X
DOIs
Publication statusPublished - Sep 2019

ID: 58286260