Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

An Equine Wound Model to Study Effects of Bacterial Aggregates on Wound Healing

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Bacterial Aggregates Establish at the Edges of Acute Epidermal Wounds

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Antibiofilm Properties of Acetic Acid

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Minimum information guideline for spectrofotometric and fluorometric methods to assess biofilm formation in microplates

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Is pseudarthrosis after spinal instrumentation caused by a chronic infection?

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Analysis of proximal bone margins in diabetic foot osteomyelitis by conventional culture, DNA sequencing and microscopy

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Antimicrobial Tolerance and Metabolic Adaptations in Microbial Biofilms

    Research output: Contribution to journalReviewResearchpeer-review

View graph of relations

Objective: Relevant animal models to study effects of bacterial aggregates on wound healing are lacking. We aimed at establishing an equine wound model with bacterial aggregates to investigate the impact of bacterial inoculation on normal (thorax) and impaired (limb) wound healing. Approach: Wounds were created on three limbs and both thorax sides of six horses. Twelve out of 20 wounds per horse were inoculated with 104Staphylococcus aureus and 105Pseudomonas aeruginosa on day 4. Healing was monitored until day 27 by clinical assessment, including wound scoring, surface pH measurements, and digital photography for area determination. Biopsies were used for bacterial culture and for peptide nucleic acid fluorescence in situ hybridization to detect bacterial aggregates. Results: Inoculated limb wounds healed slower than noninoculated limb wounds from day 10 onward (p < 0.0001). Inoculated and noninoculated thorax wounds healed equally well and faster than limb wounds. The odds ratio of detecting bacterial aggregates in inoculated limb wounds was 7.1 (2.4-21.0, p = 0.0086) compared with noninoculated limb wounds and 36.2 (3.8-348, p = 0.0018) compared with thorax wounds. Innovation: This equine wound model with bacterial aggregates might be superior to other animal wound models, as both normal and impaired healing can be studied simultaneously. In this model, many aspects of wound healing, including novel treatments, may be studied. Conclusions: The impaired healing observed in inoculated limb wounds may be related to the persistent bacterial aggregates. Both in capability of clearing inoculated bacteria from the wounds and in healing pattern, thorax wounds were superior to limb wounds.

Original languageEnglish
JournalAdvances in Wound Care
Volume8
Issue number10
Pages (from-to)487-498
Number of pages12
ISSN2162-1918
DOIs
Publication statusPublished - 1 Oct 2019

ID: 58994514