Amphiphilic γ-PGA nanoparticles administered on rat middle ear mucosa produce adjuvant-like immunostimulation in vivo

Johan Nilsson, Sissela Broos, Takami Akagi, Mitsuru Akashi, Ann Hermansson, Per Cayé-Thomasen, Malin Lindstedt, Lennart Greiff

5 Citations (Scopus)

Abstract

CONCLUSION: Amphiphilic biodegradable nanoparticles (NPs) composed of poly(γ-glutamic acid) conjugated with L-phenylalanine ethylester (γ-PGA-Phe NPs) applied on the rat middle ear mucosa produce an inflammatory type 1 response. The observation is of relevance for the use of γ-PGA-Phe NPs as a concomitant antigen delivery system and adjuvant measure in the context of vaccinations.

OBJECTIVES: To examine effects of topical mucosal administration of γ-PGA-Phe NPs as a potentially combined antigen delivery system and adjuvant.

METHODS: γ-PGA-Phe NPs were administered on rat middle ear mucosa in a sham-controlled design and the response was monitored, focusing on soluble markers in mucosal surface liquids and on overall histopathology.

RESULTS: γ-PGA-Phe NPs produced a dose- and time-dependent inflammatory response characterized by generation of proinflammatory cytokines (IL-1α, IL-1β, IL-6, MIP-1α, and TNF-α) and associated histopathological changes.

Original languageEnglish
JournalActa Oto-Laryngologica
Volume134
Issue number10
Pages (from-to)1034-41
Number of pages8
ISSN0001-6489
DOIs
Publication statusPublished - Oct 2014

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