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Alveolar recruitment of ficolin-3 in response to acute pulmonary inflammation in humans

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  1. Expression of complement C3, C5, C3aR and C5aR1 genes in resting and activated CD4+ T cells

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  2. Reconstitution of Th17, Tc17 and Treg cells after paediatric haematopoietic stem cell transplantation: Impact of interleukin-7

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  3. Angiotensin II blockade, YKL-40 and maintenance of sinus rhythm after electrical cardioversion for atrial fibrillation

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  4. Plasma YKL-40 and CHI3L1 in systemic inflammation and sepsis-Experience from two prospective cohorts

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  1. Development and validation of a multiple-choice questionnaire-based theoretical test in direct ophthalmoscopy

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  2. Decentralized virtual reality mastoidectomy simulation training: a prospective, mixed-methods study

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  3. Simulation-Based Training of Ultrasound-Guided Procedures in Radiology - A Systematic Review

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  4. Using virtual-reality simulation to ensure basic competence in hysteroscopy

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  5. Plasma levels of mannose-binding lectin and future risk of venous thromboembolism

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BACKGROUND: Ficolins serve as soluble recognition molecules in the lectin pathway of complement. They are known to participate in the systemic host-response to infection but their role in local pulmonary defence is still incompletely understood. The purpose of this study was to clarify whether acute lung and systemic inflammation induce recruitment of lectins in humans.

METHODS: Fifteen healthy volunteers received LPS intravenously (IV) or in a lung subsegment on two different occasions. Volunteers were evaluated by consecutive blood samples and by bronchoalveolar lavage 2, 4, 6, 8, or 24h after LPS (n=3 in all groups), and gene expression patterns and protein levels of mannose-binding lectin (MBL) and ficolins were determined.

RESULTS: Endobronchial LPS was associated with an increase in alveolar ficolin-3 and MBL levels (p<0.04 and p<0.001, respectively). IV LPS elicited a pronounced acute phase response with an increase in CRP (p<0.001) and plasma ficolin-1 protein levels (p<0.001), whereas no changes were observed in ficolin-1 gene expression patterns (p=0.11) or plasma protein levels of MBL, ficolin-2, or ficolin-3.

CONCLUSIONS: LPS induces a tissue-specific recruitment of ficolin-3 and ficolin-1 in the lung and systemic compartment, respectively, suggesting an important role of distinct lectin complement pathway initiators in the local pulmonary and systemic host defence.

Original languageEnglish
JournalImmunobiology
Volume221
Issue number5
Pages (from-to)690-7
Number of pages8
ISSN0171-2985
DOIs
Publication statusPublished - May 2016

ID: 46478653