All prepro-VIP-derived peptides, except PHI/PHV, are expressed in the female rat anterior pituitary and increased by estrogen

M L Skakkebaek; B Georg; J D Mikkelsen; B Ottesen; J Fahrenkrug

doi:10.1016/0196-9781(95)02010-t

All prepro-VIP-derived peptides, except PHI/PHV, are expressed in the female rat anterior pituitary and increased by estrogen

M L Skakkebaek, B Georg, J D Mikkelsen, B Ottesen, J Fahrenkrug

5 Citations (Scopus)

Abstract

The expression of VIP precursor products: prepro-VIP(22-79), peptide histidine isoleucine (PHI), peptide histidine valine (PHV), prepro-VIP(111-122), VIP, prepro-VIP(156-170), and prepro-VIP mRNA in the anterior pituitary of estrogen-treated, ovariectomized rats, of ovariectomized controls, and of sham-operated controls was examined. Using radioimmunoassays based on antisera against each of the prepro-VIP sequences, we found that all sequences were expressed and markedly induced by estrogen, except PHI and PHV, which both were undetectable. By immunohistochemistry, it appeared that the number of cells immunoreactive for each of these sequences was increased in the estrogen-treated animals. However, PHI/PHV-immunoreactive cells could not be detected, despite the use of four different PHI antisera with different specificities. Estrogen treatment increased the prepro-VIP mRNA as judged by Northern blotting. In situ hybridization signals for both VIP mRNA and PHI mRNA were observed in few pituitary cells from control animals whereas strong positive signals were observed in a larger number of cells after estrogen treatment. The findings show that estrogen causes activation of the VIP gene expression in anterior pituitary cells, and that the absence of PHI and PHV probably is due to translational or posttranslational events.

Original language	English
Journal	Peptides
Volume	16
Issue number	7
Pages (from-to)	1287-94
Number of pages	8
ISSN	0196-9781
DOIs	https://doi.org/10.1016/0196-9781(95)02010-t
Publication status	Published - 1995
Externally published	Yes

Keywords

Amino Acid Sequence
Animals
Blotting, Northern
Estrogens/pharmacology
Female
Immunohistochemistry
In Situ Hybridization
Molecular Sequence Data
Peptide Fragments/isolation & purification
Peptide PHI/isolation & purification
Pituitary Gland, Anterior/anatomy & histology
Protein Precursors/genetics
RNA, Messenger/isolation & purification
Radioimmunoassay
Rats
Rats, Wistar
Vasoactive Intestinal Peptide/genetics

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10.1016/0196-9781(95)02010-t

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@article{ee1d568bfa334d2fae110b41bd548167,

title = "All prepro-VIP-derived peptides, except PHI/PHV, are expressed in the female rat anterior pituitary and increased by estrogen",

abstract = "The expression of VIP precursor products: prepro-VIP(22-79), peptide histidine isoleucine (PHI), peptide histidine valine (PHV), prepro-VIP(111-122), VIP, prepro-VIP(156-170), and prepro-VIP mRNA in the anterior pituitary of estrogen-treated, ovariectomized rats, of ovariectomized controls, and of sham-operated controls was examined. Using radioimmunoassays based on antisera against each of the prepro-VIP sequences, we found that all sequences were expressed and markedly induced by estrogen, except PHI and PHV, which both were undetectable. By immunohistochemistry, it appeared that the number of cells immunoreactive for each of these sequences was increased in the estrogen-treated animals. However, PHI/PHV-immunoreactive cells could not be detected, despite the use of four different PHI antisera with different specificities. Estrogen treatment increased the prepro-VIP mRNA as judged by Northern blotting. In situ hybridization signals for both VIP mRNA and PHI mRNA were observed in few pituitary cells from control animals whereas strong positive signals were observed in a larger number of cells after estrogen treatment. The findings show that estrogen causes activation of the VIP gene expression in anterior pituitary cells, and that the absence of PHI and PHV probably is due to translational or posttranslational events.",

keywords = "Amino Acid Sequence, Animals, Blotting, Northern, Estrogens/pharmacology, Female, Immunohistochemistry, In Situ Hybridization, Molecular Sequence Data, Peptide Fragments/isolation \& purification, Peptide PHI/isolation \& purification, Pituitary Gland, Anterior/anatomy \& histology, Protein Precursors/genetics, RNA, Messenger/isolation \& purification, Radioimmunoassay, Rats, Rats, Wistar, Vasoactive Intestinal Peptide/genetics",

author = "Skakkebaek, \{M L\} and B Georg and Mikkelsen, \{J D\} and B Ottesen and J Fahrenkrug",

year = "1995",

doi = "10.1016/0196-9781(95)02010-t",

language = "English",

volume = "16",

pages = "1287--94",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "7",

}

TY - JOUR

T1 - All prepro-VIP-derived peptides, except PHI/PHV, are expressed in the female rat anterior pituitary and increased by estrogen

AU - Skakkebaek, M L

AU - Georg, B

AU - Mikkelsen, J D

AU - Ottesen, B

AU - Fahrenkrug, J

PY - 1995

Y1 - 1995

N2 - The expression of VIP precursor products: prepro-VIP(22-79), peptide histidine isoleucine (PHI), peptide histidine valine (PHV), prepro-VIP(111-122), VIP, prepro-VIP(156-170), and prepro-VIP mRNA in the anterior pituitary of estrogen-treated, ovariectomized rats, of ovariectomized controls, and of sham-operated controls was examined. Using radioimmunoassays based on antisera against each of the prepro-VIP sequences, we found that all sequences were expressed and markedly induced by estrogen, except PHI and PHV, which both were undetectable. By immunohistochemistry, it appeared that the number of cells immunoreactive for each of these sequences was increased in the estrogen-treated animals. However, PHI/PHV-immunoreactive cells could not be detected, despite the use of four different PHI antisera with different specificities. Estrogen treatment increased the prepro-VIP mRNA as judged by Northern blotting. In situ hybridization signals for both VIP mRNA and PHI mRNA were observed in few pituitary cells from control animals whereas strong positive signals were observed in a larger number of cells after estrogen treatment. The findings show that estrogen causes activation of the VIP gene expression in anterior pituitary cells, and that the absence of PHI and PHV probably is due to translational or posttranslational events.

AB - The expression of VIP precursor products: prepro-VIP(22-79), peptide histidine isoleucine (PHI), peptide histidine valine (PHV), prepro-VIP(111-122), VIP, prepro-VIP(156-170), and prepro-VIP mRNA in the anterior pituitary of estrogen-treated, ovariectomized rats, of ovariectomized controls, and of sham-operated controls was examined. Using radioimmunoassays based on antisera against each of the prepro-VIP sequences, we found that all sequences were expressed and markedly induced by estrogen, except PHI and PHV, which both were undetectable. By immunohistochemistry, it appeared that the number of cells immunoreactive for each of these sequences was increased in the estrogen-treated animals. However, PHI/PHV-immunoreactive cells could not be detected, despite the use of four different PHI antisera with different specificities. Estrogen treatment increased the prepro-VIP mRNA as judged by Northern blotting. In situ hybridization signals for both VIP mRNA and PHI mRNA were observed in few pituitary cells from control animals whereas strong positive signals were observed in a larger number of cells after estrogen treatment. The findings show that estrogen causes activation of the VIP gene expression in anterior pituitary cells, and that the absence of PHI and PHV probably is due to translational or posttranslational events.

KW - Amino Acid Sequence

KW - Animals

KW - Blotting, Northern

KW - Estrogens/pharmacology

KW - Female

KW - Immunohistochemistry

KW - In Situ Hybridization

KW - Molecular Sequence Data

KW - Peptide Fragments/isolation & purification

KW - Peptide PHI/isolation & purification

KW - Pituitary Gland, Anterior/anatomy & histology

KW - Protein Precursors/genetics

KW - RNA, Messenger/isolation & purification

KW - Radioimmunoassay

KW - Rats

KW - Rats, Wistar

KW - Vasoactive Intestinal Peptide/genetics

UR - https://www.scopus.com/pages/publications/0028849424

U2 - 10.1016/0196-9781(95)02010-t

DO - 10.1016/0196-9781(95)02010-t

M3 - Journal article

C2 - 8545253

SN - 0196-9781

VL - 16

SP - 1287

EP - 1294

JO - Peptides

JF - Peptides

IS - 7

ER -

All prepro-VIP-derived peptides, except PHI/PHV, are expressed in the female rat anterior pituitary and increased by estrogen

Abstract

Keywords

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