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Airway Interleukin-33 and type 2 cytokines in adult patients with acute asthma

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Poulsen, Nadia Nicholine ; Bjerregaard, Asger ; Khoo, Siew-Kim ; Laing, Ingrid A ; Le Souëf, Peter ; Backer, Vibeke ; Rapley, Laura ; Cohen, Suzanne E ; Barrett, Lucy ; Thompson, Philip ; Baltic, Svetlana ; Porsbjerg, Celeste. / Airway Interleukin-33 and type 2 cytokines in adult patients with acute asthma. In: Respiratory medicine. 2018 ; Vol. 140. pp. 50-56.

Bibtex

@article{8aa1249181124f86839d6bf7ae55bb35,
title = "Airway Interleukin-33 and type 2 cytokines in adult patients with acute asthma",
abstract = "BACKGROUND: Several animal studies, and one inoculation study in adult asthmatics have shown that interleukin-33 (IL-33) is a major contributor to type-2 inflammation in acute asthma. However, the link between IL-33 and type-2 inflammation has not been shown in naturally occurring asthma exacerbations.OBJECTIVES: To determine if airway IL-33 is associated with type-2 inflammation measured by type-2 cytokines, FeNO and sputum eosinophils in patients presenting to the Emergency Department with an asthma exacerbations.METHODS: Adult patients hospitalized due to acute asthma were enrolled. Upper airways were sampled with nasal swabs and lower airways with induced sputum. Cytokines were measured at protein level using a Luminex{\circledR} assay and mRNA expression level using droplet-digital-PCR. Airway sampling was repeated four weeks after exacerbation.RESULTS: At the time of exacerbation, upper airway IL-33 correlated with upper airway IL-5 and IL-13 (R = 0.84, p < 0.01 and R = 0.76, p < 0.01, respectively) and with lower airway IL-13 (R = 0.49, p = 0.03). Similar associations were observed for mRNA expression. Lower airway IL-33 positively correlated with lower airway IL-13 (R = 0.84, p < 0.01). IL-13 and IL-33 were positively correlated with FeNO, and IL-5 with eosinophils. The association between IL-33 and type-2 cytokines were still present four weeks after exacerbation.CONCLUSION: This is the first study to demonstrate that airway IL-33 is associated with type-2 cytokines in naturally occurring asthma exacerbations in adults, providing in vivo evidence supporting that IL-33 may be driving type-2 inflammation in acute asthma. Thus supporting IL-33 as a potential future drug target due to its role, upstream in the immunological cascade.",
author = "Poulsen, {Nadia Nicholine} and Asger Bjerregaard and Siew-Kim Khoo and Laing, {Ingrid A} and {Le Sou{\"e}f}, Peter and Vibeke Backer and Laura Rapley and Cohen, {Suzanne E} and Lucy Barrett and Philip Thompson and Svetlana Baltic and Celeste Porsbjerg",
note = "Copyright {\circledC} 2018 Elsevier Ltd. All rights reserved.",
year = "2018",
month = "7",
doi = "10.1016/j.rmed.2018.05.016",
language = "English",
volume = "140",
pages = "50--56",
journal = "Respiratory medicine",
issn = "0954-6111",
publisher = "W.B./Saunders Co. Ltd",

}

RIS

TY - JOUR

T1 - Airway Interleukin-33 and type 2 cytokines in adult patients with acute asthma

AU - Poulsen, Nadia Nicholine

AU - Bjerregaard, Asger

AU - Khoo, Siew-Kim

AU - Laing, Ingrid A

AU - Le Souëf, Peter

AU - Backer, Vibeke

AU - Rapley, Laura

AU - Cohen, Suzanne E

AU - Barrett, Lucy

AU - Thompson, Philip

AU - Baltic, Svetlana

AU - Porsbjerg, Celeste

N1 - Copyright © 2018 Elsevier Ltd. All rights reserved.

PY - 2018/7

Y1 - 2018/7

N2 - BACKGROUND: Several animal studies, and one inoculation study in adult asthmatics have shown that interleukin-33 (IL-33) is a major contributor to type-2 inflammation in acute asthma. However, the link between IL-33 and type-2 inflammation has not been shown in naturally occurring asthma exacerbations.OBJECTIVES: To determine if airway IL-33 is associated with type-2 inflammation measured by type-2 cytokines, FeNO and sputum eosinophils in patients presenting to the Emergency Department with an asthma exacerbations.METHODS: Adult patients hospitalized due to acute asthma were enrolled. Upper airways were sampled with nasal swabs and lower airways with induced sputum. Cytokines were measured at protein level using a Luminex® assay and mRNA expression level using droplet-digital-PCR. Airway sampling was repeated four weeks after exacerbation.RESULTS: At the time of exacerbation, upper airway IL-33 correlated with upper airway IL-5 and IL-13 (R = 0.84, p < 0.01 and R = 0.76, p < 0.01, respectively) and with lower airway IL-13 (R = 0.49, p = 0.03). Similar associations were observed for mRNA expression. Lower airway IL-33 positively correlated with lower airway IL-13 (R = 0.84, p < 0.01). IL-13 and IL-33 were positively correlated with FeNO, and IL-5 with eosinophils. The association between IL-33 and type-2 cytokines were still present four weeks after exacerbation.CONCLUSION: This is the first study to demonstrate that airway IL-33 is associated with type-2 cytokines in naturally occurring asthma exacerbations in adults, providing in vivo evidence supporting that IL-33 may be driving type-2 inflammation in acute asthma. Thus supporting IL-33 as a potential future drug target due to its role, upstream in the immunological cascade.

AB - BACKGROUND: Several animal studies, and one inoculation study in adult asthmatics have shown that interleukin-33 (IL-33) is a major contributor to type-2 inflammation in acute asthma. However, the link between IL-33 and type-2 inflammation has not been shown in naturally occurring asthma exacerbations.OBJECTIVES: To determine if airway IL-33 is associated with type-2 inflammation measured by type-2 cytokines, FeNO and sputum eosinophils in patients presenting to the Emergency Department with an asthma exacerbations.METHODS: Adult patients hospitalized due to acute asthma were enrolled. Upper airways were sampled with nasal swabs and lower airways with induced sputum. Cytokines were measured at protein level using a Luminex® assay and mRNA expression level using droplet-digital-PCR. Airway sampling was repeated four weeks after exacerbation.RESULTS: At the time of exacerbation, upper airway IL-33 correlated with upper airway IL-5 and IL-13 (R = 0.84, p < 0.01 and R = 0.76, p < 0.01, respectively) and with lower airway IL-13 (R = 0.49, p = 0.03). Similar associations were observed for mRNA expression. Lower airway IL-33 positively correlated with lower airway IL-13 (R = 0.84, p < 0.01). IL-13 and IL-33 were positively correlated with FeNO, and IL-5 with eosinophils. The association between IL-33 and type-2 cytokines were still present four weeks after exacerbation.CONCLUSION: This is the first study to demonstrate that airway IL-33 is associated with type-2 cytokines in naturally occurring asthma exacerbations in adults, providing in vivo evidence supporting that IL-33 may be driving type-2 inflammation in acute asthma. Thus supporting IL-33 as a potential future drug target due to its role, upstream in the immunological cascade.

U2 - 10.1016/j.rmed.2018.05.016

DO - 10.1016/j.rmed.2018.05.016

M3 - Journal article

VL - 140

SP - 50

EP - 56

JO - Respiratory medicine

JF - Respiratory medicine

SN - 0954-6111

ER -

ID: 54707243