Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Age and prior exercise in vivo determine the subsequent in vitro molecular profile of myoblasts and nonmyogenic cells derived from human skeletal muscle

Research output: Contribution to journalJournal articleResearchpeer-review

  1. Activation of the TASK-2 channel after cell swelling is dependent on tyrosine phosphorylation

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Monovalent ions control proliferation of Ehrlich Lettre ascites cells

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Maintenance of muscle strength following a one-year resistance training program in older adults

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. The influence of prolonged strength training upon muscle and fat in healthy and chronically diseased older adults

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Cardiovascular and metabolic health effects of team handball training in overweight women: Impact of prior experience

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. The effect of 4 months exercise training on systemic biomarkers of cartilage and bone turnover in hip osteoarthritis patients

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Early development of tendinopathy in humans: Sequence of pathological changes in structure and tissue turnover signaling

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

The decline in skeletal muscle regenerative capacity with age is partly attributed to muscle stem cell (satellite cell) dysfunction. Recent evidence has pointed to a strong interaction between myoblasts and fibroblasts, but the influence of age on this interaction is unknown. Additionally, while the native tissue environment is known to determine the properties of myogenic cells in vitro, how the aging process alters this cell memory has not been established at the molecular level. We recruited 12 young and 12 elderly women, who performed a single bout of heavy resistance exercise with the knee extensor muscles of one leg. Five days later, muscle biopsies were collected from both legs, and myogenic cells and nonmyogenic cells were isolated for in vitro experiments with mixed or separated cells and analyzed by immunostaining and RT-PCR. A lower myogenic fusion index was detected in the cells from the old versus young women, in association with differences in gene expression levels of key myogenic regulatory factors and senescence, which were further altered by performing exercise before tissue sampling. Coculture with nonmyogenic cells from the elderly led to a higher myogenic differentiation index compared with nonmyogenic cells from the young. These findings show that the in vitro phenotype and molecular profile of human skeletal muscle myoblasts and fibroblasts is determined by the age and exercise state of the original in vivo environment and help explain how exercise can enhance muscle stem cell function in old age.

Original languageEnglish
JournalAmerican Journal of Physiology: Cell Physiology
Volume316
Issue number6
Pages (from-to)C898-C912
ISSN0363-6143
DOIs
Publication statusPublished - 1 Jun 2019

    Research areas

  • Adult, Aged, Aging/metabolism, Cells, Cultured, Coculture Techniques, Exercise/physiology, Female, Fibroblasts/metabolism, Humans, Muscle Development/physiology, Muscle, Skeletal/cytology, Myoblasts, Skeletal/metabolism, Young Adult

ID: 59361599