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Advances in capillary electrochromatography and micro-high performance liquid chromatography monolithic columns for separation science

Cristina Legido-Quigley, Nicola D Marlin, Virginie Melin, Andreas Manz, Norman W Smith

218 Citations (Scopus)

Abstract

Over the last decade, monoliths or continuous beds have emerged as an alternative to traditional packed-bed columns for use in capillary electrochromatography (CEC) and micro-high performance liquid chromatography (micro-HPLC). Monolithic columns can be divided into two categories: silica-based monolithic columns and rigid organic polymer-based monolithic columns resulting from the polymerization of acrylamide, styrene, acrylate or methacrylate monomers. In this paper, the chemistry and most recent applications of these various types of monoliths in both CEC and micro-HPLC are presented.

Original languageEnglish
JournalElectrophoresis
Volume24
Issue number6
Pages (from-to)917-44
Number of pages28
ISSN0173-0835
DOIs
Publication statusPublished - Mar 2003
Externally publishedYes

Keywords

  • Acrylates/chemistry
  • Acrylic Resins/chemistry
  • Chromatography, High Pressure Liquid/methods
  • Electrophoresis, Capillary/methods
  • Phase Transition
  • Polystyrenes/chemistry

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