Acute retinal ischemia caused by controlled low ocular perfusion pressure in a porcine model. Electrophysiological and histological characterisation

Maria Voss Kyhn, Karin Warfvinge, Erik Scherfig, Jens F Kiilgaard, Jan Ulrik Prause, Henry Klassen, Michael Young, Morten la Cour

22 Citations (Scopus)

Abstract

The purpose of this study was to establish, and characterize a porcine model of acute, controlled retinal ischemia. The controlled retinal ischemia was produced by clamping the ocular perfusion pressure (OPP) in the left eye to 5 mm Hg for 2 h. The OPP was defined as mean arterial blood pressure (MAP) minus the intraocular pressure (IOP). It was clamped to 0-30 mm Hg by continuous monitoring of MAP and adjustment of the IOP, which was controlled by cannulation of the anterior chamber. Inner retinal function was assessed by induced multifocal electroretinography (mfERG) with comparisons of the amplitudes obtained in the experimental, left eye, and the control, right eye. Quantitative histology was performed to measure the survival of ganglion cells, amacrine cells and horizontal cells 2-6 weeks after the ischemic insult. An OPP of 5 mm Hg for 2h induced significant reductions in the amplitudes of iN1 to 20% (CI: 13-30%), and iP2 to 14% (95% CI: 8-22%) of their baseline values. No signs of recovery were found within the 6-week observation period. Quantitative histology revealed a highly significant reduction in the number of ganglion cells, amacrine cells and horizontal cells after the ischemic insult. This model seems to be suitable for investigations of therapeutic initiatives in diseases involving acute retinal ischemia.
Original languageEnglish
JournalExperimental Eye Research
Volume88
Issue number6
Pages (from-to)1100-6
Number of pages7
ISSN0014-4835
DOIs
Publication statusPublished - 1 Jun 2009

Keywords

  • Acute Disease
  • Amacrine Cells
  • Animals
  • Blood Pressure
  • Disease Models, Animal
  • Electroretinography
  • Female
  • Intraocular Pressure
  • Ischemia
  • Retinal Diseases
  • Retinal Ganglion Cells
  • Retinal Horizontal Cells
  • Retinal Vessels
  • Sus scrofa

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