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Acute administration of interleukin-6 does not increase secretion of glucagon-like peptide-1 in mice

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Harvard

Christiansen, CB, Lind, SJ, Svendsen, B, Balk-Møller, E, Dahlby, T, Kuhre, RE, Hartmann, B, Mandrup-Poulsen, T, Deacon, CF, Wewer Albrechtsen, NJ & Holst, JJ 2018, 'Acute administration of interleukin-6 does not increase secretion of glucagon-like peptide-1 in mice' Physiological Reports, vol. 6, no. 13, pp. e13788. https://doi.org/10.14814/phy2.13788

APA

Christiansen, C. B., Lind, S. J., Svendsen, B., Balk-Møller, E., Dahlby, T., Kuhre, R. E., ... Holst, J. J. (2018). Acute administration of interleukin-6 does not increase secretion of glucagon-like peptide-1 in mice. Physiological Reports, 6(13), e13788. https://doi.org/10.14814/phy2.13788

CBE

Christiansen CB, Lind SJ, Svendsen B, Balk-Møller E, Dahlby T, Kuhre RE, Hartmann B, Mandrup-Poulsen T, Deacon CF, Wewer Albrechtsen NJ, Holst JJ. 2018. Acute administration of interleukin-6 does not increase secretion of glucagon-like peptide-1 in mice. Physiological Reports. 6(13):e13788. https://doi.org/10.14814/phy2.13788

MLA

Vancouver

Christiansen CB, Lind SJ, Svendsen B, Balk-Møller E, Dahlby T, Kuhre RE et al. Acute administration of interleukin-6 does not increase secretion of glucagon-like peptide-1 in mice. Physiological Reports. 2018 Jul;6(13):e13788. https://doi.org/10.14814/phy2.13788

Author

Christiansen, Charlotte B ; Lind, Sara J ; Svendsen, Berit ; Balk-Møller, Emilie ; Dahlby, Tina ; Kuhre, Rune E ; Hartmann, Bolette ; Mandrup-Poulsen, Thomas ; Deacon, Carolyn F ; Wewer Albrechtsen, Nicolai J ; Holst, Jens J. / Acute administration of interleukin-6 does not increase secretion of glucagon-like peptide-1 in mice. In: Physiological Reports. 2018 ; Vol. 6, No. 13. pp. e13788.

Bibtex

@article{56dbb6678d8549b9b5a58acbad75f3cb,
title = "Acute administration of interleukin-6 does not increase secretion of glucagon-like peptide-1 in mice",
abstract = "Interleukin 6 (IL-6) is a cytokine secreted from skeletal muscle in response to exercise which, based on animal and cell studies, has been suggested to contribute to glucose metabolism by increasing secretion of the incretin hormone glucagon-like peptide-1 (GLP-1) and affecting secretion of insulin and glucagon from the pancreatic islets. We investigated the effect of IL-6 on GLP-1 secretion in GLP-1 producing cells (GLUTag) and using the perfused mouse small intestine (harboring GLP-1 producing cells). Furthermore, the direct effect of IL-6 on insulin and glucagon secretion was studied using isolated perfused mouse pancreas. Incubating GLUTag cells with 1000 ng/mL of IL-6 for 2 h did not significantly increase secretion of GLP-1 whereas 10 mmol/L glucose (positive control) did. Similarly, IL-6 (100 ng/mL) had no effect on GLP-1 secretion from perfused mouse small intestine whereas bombesin (positive control) increased secretion. Finally, administering IL-6 (100 ng/mL) to perfused mouse pancreases did not significantly increase insulin or glucagon secretion regardless of perfusate glucose levels (3.5 vs. 12 mmol/L glucose). Acute effects of IL-6 therefore do not seem to include a stimulatory effect on GLP-1 secretion in mice.",
author = "Christiansen, {Charlotte B} and Lind, {Sara J} and Berit Svendsen and Emilie Balk-M{\o}ller and Tina Dahlby and Kuhre, {Rune E} and Bolette Hartmann and Thomas Mandrup-Poulsen and Deacon, {Carolyn F} and {Wewer Albrechtsen}, {Nicolai J} and Holst, {Jens J}",
note = "{\circledC} 2018 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.",
year = "2018",
month = "7",
doi = "10.14814/phy2.13788",
language = "English",
volume = "6",
pages = "e13788",
journal = "Physiological Reports",
issn = "2051-817X",
publisher = "John Wiley and Sons Inc.",
number = "13",

}

RIS

TY - JOUR

T1 - Acute administration of interleukin-6 does not increase secretion of glucagon-like peptide-1 in mice

AU - Christiansen, Charlotte B

AU - Lind, Sara J

AU - Svendsen, Berit

AU - Balk-Møller, Emilie

AU - Dahlby, Tina

AU - Kuhre, Rune E

AU - Hartmann, Bolette

AU - Mandrup-Poulsen, Thomas

AU - Deacon, Carolyn F

AU - Wewer Albrechtsen, Nicolai J

AU - Holst, Jens J

N1 - © 2018 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.

PY - 2018/7

Y1 - 2018/7

N2 - Interleukin 6 (IL-6) is a cytokine secreted from skeletal muscle in response to exercise which, based on animal and cell studies, has been suggested to contribute to glucose metabolism by increasing secretion of the incretin hormone glucagon-like peptide-1 (GLP-1) and affecting secretion of insulin and glucagon from the pancreatic islets. We investigated the effect of IL-6 on GLP-1 secretion in GLP-1 producing cells (GLUTag) and using the perfused mouse small intestine (harboring GLP-1 producing cells). Furthermore, the direct effect of IL-6 on insulin and glucagon secretion was studied using isolated perfused mouse pancreas. Incubating GLUTag cells with 1000 ng/mL of IL-6 for 2 h did not significantly increase secretion of GLP-1 whereas 10 mmol/L glucose (positive control) did. Similarly, IL-6 (100 ng/mL) had no effect on GLP-1 secretion from perfused mouse small intestine whereas bombesin (positive control) increased secretion. Finally, administering IL-6 (100 ng/mL) to perfused mouse pancreases did not significantly increase insulin or glucagon secretion regardless of perfusate glucose levels (3.5 vs. 12 mmol/L glucose). Acute effects of IL-6 therefore do not seem to include a stimulatory effect on GLP-1 secretion in mice.

AB - Interleukin 6 (IL-6) is a cytokine secreted from skeletal muscle in response to exercise which, based on animal and cell studies, has been suggested to contribute to glucose metabolism by increasing secretion of the incretin hormone glucagon-like peptide-1 (GLP-1) and affecting secretion of insulin and glucagon from the pancreatic islets. We investigated the effect of IL-6 on GLP-1 secretion in GLP-1 producing cells (GLUTag) and using the perfused mouse small intestine (harboring GLP-1 producing cells). Furthermore, the direct effect of IL-6 on insulin and glucagon secretion was studied using isolated perfused mouse pancreas. Incubating GLUTag cells with 1000 ng/mL of IL-6 for 2 h did not significantly increase secretion of GLP-1 whereas 10 mmol/L glucose (positive control) did. Similarly, IL-6 (100 ng/mL) had no effect on GLP-1 secretion from perfused mouse small intestine whereas bombesin (positive control) increased secretion. Finally, administering IL-6 (100 ng/mL) to perfused mouse pancreases did not significantly increase insulin or glucagon secretion regardless of perfusate glucose levels (3.5 vs. 12 mmol/L glucose). Acute effects of IL-6 therefore do not seem to include a stimulatory effect on GLP-1 secretion in mice.

U2 - 10.14814/phy2.13788

DO - 10.14814/phy2.13788

M3 - Journal article

VL - 6

SP - e13788

JO - Physiological Reports

JF - Physiological Reports

SN - 2051-817X

IS - 13

ER -

ID: 55761858