Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Activation of satellite cells and the regeneration of human skeletal muscle are expedited by ingestion of nonsteroidal anti-inflammatory medication

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Influence of FGF23 and Klotho on male reproduction: Systemic vs direct effects

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Prenatal inflammation suppresses blood Th1 polarization and gene clusters related to cellular energy metabolism in preterm newborns

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Early development of tendinopathy in humans: Sequence of pathological changes in structure and tissue turnover signaling

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Maintenance of muscle strength following a one-year resistance training program in older adults

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. The influence of prolonged strength training upon muscle and fat in healthy and chronically diseased older adults

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. The effect of 4 months exercise training on systemic biomarkers of cartilage and bone turnover in hip osteoarthritis patients

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Early development of tendinopathy in humans: Sequence of pathological changes in structure and tissue turnover signaling

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. The influence of fibrillin-1 and physical activity upon tendon tissue morphology and mechanical properties in mice

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

With this study we investigated the role of nonsteroidal anti-inflammatory drugs (NSAIDs) in human skeletal muscle regeneration. Young men ingested NSAID [1200 mg/d ibuprofen (IBU)] or placebo (PLA) daily for 2 wk before and 4 wk after an electrical stimulation-induced injury to the leg extensor muscles of one leg. Muscle biopsies were collected from the vastus lateralis muscles before and after stimulation (2.5 h and 2, 7, and 30 d) and were assessed for satellite cells and regeneration by immunohistochemistry and real-time RT-PCR, and we also measured telomere length. After injury, and compared with PLA, IBU was found to augment the proportion of ActiveNotch1(+) satellite cells at 2 d [IBU, 29 ± 3% vs. PLA, 19 ± 2% (means ± sem)], satellite cell content at 7 d [IBU, 0.16 ± 0.01 vs. PLA, 0.12 ± 0.01 (Pax7(+) cells/fiber)], and to expedite muscle repair at 30 d. The PLA group displayed a greater proportion of embryonic myosin(+) fibers and a residual ∼2-fold increase in mRNA levels of matrix proteins (all P < 0.05). Endomysial collagen was also elevated with PLA at 30 d. Minimum telomere length shortening was not observed. In conclusion, ingestion of NSAID has a potentiating effect on Notch activation of satellite cells and muscle remodeling during large-scale regeneration of injured human skeletal muscle.-Mackey, A. L., Rasmussen, L. K., Kadi, F., Schjerling, P., Helmark, I. C., Ponsot, E., Aagaard, P., Durigan, J. L. Q., Kjaer, M. Activation of satellite cells and the regeneration of human skeletal muscle are expedited by ingestion of nonsteroidal anti-inflammatory medication.

Original languageEnglish
JournalFASEB Journal
Volume30
Issue number6
Pages (from-to)2266-81
Number of pages16
ISSN0892-6638
DOIs
Publication statusPublished - Jun 2016

    Research areas

  • Journal Article

ID: 49634174