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Acquisition of IgG to ICAM-1-Binding DBLβ Domains in the Plasmodium falciparum Erythrocyte Membrane Protein 1 Antigen Family Varies between Groups A, B, and C

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@article{edf11b51bcce493f9bbc3c4a8550e57b,
title = "Acquisition of IgG to ICAM-1-Binding DBLβ Domains in the Plasmodium falciparum Erythrocyte Membrane Protein 1 Antigen Family Varies between Groups A, B, and C",
abstract = "Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) is an important malaria virulence factor. The protein family can be divided into clinically relevant subfamilies. ICAM-1-binding group A PfEMP1 proteins also bind endothelial protein C receptor and have been associated with cerebral malaria in children. IgG to these PfEMP1 proteins is acquired later in life than that to group A PfEMP1 not binding ICAM-1. The kinetics of acquisition of IgG to group B and C PfEMP1 proteins binding ICAM-1 is unclear and was studied here. Gene sequences encoding group B and C PfEMP1 with DBLβ domains known to bind ICAM-1 were used to identify additional binders. Levels of IgG specific for DBLβ domains from group A, B, and C PfEMP1 binding or not binding ICAM-1 were measured in plasma from Ghanaian children with or without malaria. Seven new ICAM-1-binding DBLβ domains from group B and C PfEMP1 were identified. Healthy children had higher levels of IgG specific for ICAM-1-binding DBLβ domains from group A than from groups B and C. However, the opposite pattern was found in children with malaria, particularly among young patients. Acquisition of IgG specific for DBLβ domains binding ICAM-1 differs between PfEMP1 groups.",
keywords = "Antibodies, Protozoan/biosynthesis, Child, Child, Preschool, Erythrocytes/immunology, Female, Gene Expression, Ghana, Humans, Immunoglobulin G/biosynthesis, Infant, Intercellular Adhesion Molecule-1/genetics, Malaria, Cerebral/genetics, Malaria, Falciparum/genetics, Male, Plasmodium falciparum/immunology, Polymorphism, Genetic, Protein Binding, Protein Domains, Protozoan Proteins/classification, Seasons, Severity of Illness Index",
author = "Olsen, {Rebecca W} and Gertrude Ecklu-Mensah and Anja Bengtsson and Ofori, {Michael F} and Kusi, {Kwadwo A} and Koram, {Kwadwo A} and Lars Hviid and Yvonne Adams and Jensen, {Anja T R}",
note = "Copyright {\circledC} 2019 Olsen et al.",
year = "2019",
month = "10",
doi = "10.1128/IAI.00224-19",
language = "English",
volume = "87",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "10",

}

RIS

TY - JOUR

T1 - Acquisition of IgG to ICAM-1-Binding DBLβ Domains in the Plasmodium falciparum Erythrocyte Membrane Protein 1 Antigen Family Varies between Groups A, B, and C

AU - Olsen, Rebecca W

AU - Ecklu-Mensah, Gertrude

AU - Bengtsson, Anja

AU - Ofori, Michael F

AU - Kusi, Kwadwo A

AU - Koram, Kwadwo A

AU - Hviid, Lars

AU - Adams, Yvonne

AU - Jensen, Anja T R

N1 - Copyright © 2019 Olsen et al.

PY - 2019/10

Y1 - 2019/10

N2 - Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) is an important malaria virulence factor. The protein family can be divided into clinically relevant subfamilies. ICAM-1-binding group A PfEMP1 proteins also bind endothelial protein C receptor and have been associated with cerebral malaria in children. IgG to these PfEMP1 proteins is acquired later in life than that to group A PfEMP1 not binding ICAM-1. The kinetics of acquisition of IgG to group B and C PfEMP1 proteins binding ICAM-1 is unclear and was studied here. Gene sequences encoding group B and C PfEMP1 with DBLβ domains known to bind ICAM-1 were used to identify additional binders. Levels of IgG specific for DBLβ domains from group A, B, and C PfEMP1 binding or not binding ICAM-1 were measured in plasma from Ghanaian children with or without malaria. Seven new ICAM-1-binding DBLβ domains from group B and C PfEMP1 were identified. Healthy children had higher levels of IgG specific for ICAM-1-binding DBLβ domains from group A than from groups B and C. However, the opposite pattern was found in children with malaria, particularly among young patients. Acquisition of IgG specific for DBLβ domains binding ICAM-1 differs between PfEMP1 groups.

AB - Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) is an important malaria virulence factor. The protein family can be divided into clinically relevant subfamilies. ICAM-1-binding group A PfEMP1 proteins also bind endothelial protein C receptor and have been associated with cerebral malaria in children. IgG to these PfEMP1 proteins is acquired later in life than that to group A PfEMP1 not binding ICAM-1. The kinetics of acquisition of IgG to group B and C PfEMP1 proteins binding ICAM-1 is unclear and was studied here. Gene sequences encoding group B and C PfEMP1 with DBLβ domains known to bind ICAM-1 were used to identify additional binders. Levels of IgG specific for DBLβ domains from group A, B, and C PfEMP1 binding or not binding ICAM-1 were measured in plasma from Ghanaian children with or without malaria. Seven new ICAM-1-binding DBLβ domains from group B and C PfEMP1 were identified. Healthy children had higher levels of IgG specific for ICAM-1-binding DBLβ domains from group A than from groups B and C. However, the opposite pattern was found in children with malaria, particularly among young patients. Acquisition of IgG specific for DBLβ domains binding ICAM-1 differs between PfEMP1 groups.

KW - Antibodies, Protozoan/biosynthesis

KW - Child

KW - Child, Preschool

KW - Erythrocytes/immunology

KW - Female

KW - Gene Expression

KW - Ghana

KW - Humans

KW - Immunoglobulin G/biosynthesis

KW - Infant

KW - Intercellular Adhesion Molecule-1/genetics

KW - Malaria, Cerebral/genetics

KW - Malaria, Falciparum/genetics

KW - Male

KW - Plasmodium falciparum/immunology

KW - Polymorphism, Genetic

KW - Protein Binding

KW - Protein Domains

KW - Protozoan Proteins/classification

KW - Seasons

KW - Severity of Illness Index

U2 - 10.1128/IAI.00224-19

DO - 10.1128/IAI.00224-19

M3 - Journal article

VL - 87

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 10

ER -

ID: 59421092