TY - JOUR
T1 - Absence of p.R50X Pygm read-through in McArdle disease cellular models
AU - Tarrasó, Guillermo
AU - Real-Martinez, Alberto
AU - Parés, Marta
AU - Romero-Cortadellas, Lídia
AU - Puigros, Laura
AU - Moya, Laura
AU - de Luna, Noemí
AU - Brull, Astrid
AU - Martín, Miguel Angel
AU - Arenas, Joaquin
AU - Lucia, Alejandro
AU - Andreu, Antoni L
AU - Barquinero, Jordi
AU - Vissing, John
AU - Krag, Thomas O
AU - Pinós, Tomàs
N1 - © 2020. Published by The Company of Biologists Ltd.
PY - 2020/1/13
Y1 - 2020/1/13
N2 - McArdle disease is an autosomal recessive disorder caused by the absence of muscle glycogen phosphorylase, which leads to blocked muscle glycogen breakdown. We used three different cellular models to evaluate the efficiency of different read-through agents (including amlexanox, Ataluren, RTC13 and G418) in McArdle disease. The first model consisted of HeLa cells transfected with two different GFP-PYGM constructs presenting the Pygm p.R50X mutation (GFP-PYGM p.R50X and PYGM Ex1-GFP p.R50X). The second cellular model was based on the creation of HEK293T cell lines stably expressing the PYGM Ex1-GFP p.R50X construct. As these plasmids encode murine Pygm cDNA without any intron sequence, their transfection in cells would allow for analysis of the efficacy of read-through agents with no concomitant nonsense-mediated decay interference. The third model consisted of skeletal muscle cultures derived from the McArdle mouse model (knock-in for the p.R50X mutation in the Pygm gene). We found no evidence of read-through at detectable levels in any of the models evaluated. We performed a literature search and compared the premature termination codon context sequences with reported positive and negative read-through induction, identifying a potential role for nucleotide positions -9, -8, -3, -2, +13 and +14 (the first nucleotide of the stop codon is assigned as +1). The Pygm p.R50X mutation presents TGA as a stop codon, G nucleotides at positions -1 and -9, and a C nucleotide at -3, which potentially generate a good context for read-through induction, counteracted by the presence of C at -2 and its absence at +4.
AB - McArdle disease is an autosomal recessive disorder caused by the absence of muscle glycogen phosphorylase, which leads to blocked muscle glycogen breakdown. We used three different cellular models to evaluate the efficiency of different read-through agents (including amlexanox, Ataluren, RTC13 and G418) in McArdle disease. The first model consisted of HeLa cells transfected with two different GFP-PYGM constructs presenting the Pygm p.R50X mutation (GFP-PYGM p.R50X and PYGM Ex1-GFP p.R50X). The second cellular model was based on the creation of HEK293T cell lines stably expressing the PYGM Ex1-GFP p.R50X construct. As these plasmids encode murine Pygm cDNA without any intron sequence, their transfection in cells would allow for analysis of the efficacy of read-through agents with no concomitant nonsense-mediated decay interference. The third model consisted of skeletal muscle cultures derived from the McArdle mouse model (knock-in for the p.R50X mutation in the Pygm gene). We found no evidence of read-through at detectable levels in any of the models evaluated. We performed a literature search and compared the premature termination codon context sequences with reported positive and negative read-through induction, identifying a potential role for nucleotide positions -9, -8, -3, -2, +13 and +14 (the first nucleotide of the stop codon is assigned as +1). The Pygm p.R50X mutation presents TGA as a stop codon, G nucleotides at positions -1 and -9, and a C nucleotide at -3, which potentially generate a good context for read-through induction, counteracted by the presence of C at -2 and its absence at +4.
KW - Cellular models
KW - McArdle disease
KW - Metabolic myopathy
KW - Premature termination codon
KW - Read-through
UR - http://www.scopus.com/inward/record.url?scp=85078555344&partnerID=8YFLogxK
U2 - 10.1242/dmm.043281
DO - 10.1242/dmm.043281
M3 - Journal article
C2 - 31848135
SN - 1754-8403
VL - 13
SP - dmm043281.
JO - Disease models & mechanisms
JF - Disease models & mechanisms
IS - 1
M1 - dmm043281
ER -