Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

A synoviocyte model for osteoarthritis and rheumatoid arthritis: response to Ibuprofen, betamethasone, and ginger extract-a cross-sectional in vitro study

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Adherence to methotrexate in rheumatoid arthritis: a danish nationwide cohort study

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Antibodies to infliximab and adalimumab in patients with rheumatoid arthritis in clinical remission: a cross-sectional study

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Pain Sensitisation in Women with Active Rheumatoid Arthritis: A Comparative Cross-Sectional Study

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Weight-bearing MRI of the Lumbar Spine: Spinal Stenosis and Spondylolisthesis

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Weight-bearing MRI of the Lumbar Spine: Technical Aspects

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations
This study aimed at determining if synovial cell cultures from rheumatoid arthritis (RA), osteoarthritis (OA), and healthy controls (HC) differ and are suitable disease models in pharmacological studies, and tested their response to some anti-inflammatory drugs. Synovial cells were isolated from synovial membrane or joint fluid. Cells were cultivated and exposed to no or TNF-α stimulation without, or in the presence of, betamethasone, ibuprofen, or a standardized ginger extract. Concentrations of a panel of cytokines, growth factors, and chemokines were mapped for each culture and condition. Our cells secreted an increased amount of the cytokines IL-1β, IL-6, and IL-8 in response to TNF-α stimulation in all conditions. OA cells showed a higher IL-6 and IL-8 and a lower IL-1β production, when not stimulated, than RA and HC cells, which were similar. TNF-α stimulation caused similar IL-1β, IL-6, and IL-8 release in all groups. Ibuprofen showed no effect on cytokine production, while ginger extract was similar to betamethasone. Ginger extract was as effective an anti-inflammatory agent as betamethasone in this in vitro model. Cultured fibroblast-like synoviocytes from OA and RA subjects promise to be a useful pharmacological disease model, but further studies, to support results from such a model are needed.
Original languageEnglish
JournalArthritis
Volume2012
Pages (from-to)505842
DOIs
Publication statusPublished - 2012

ID: 36796078