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A radio-high-performance liquid chromatography dual-flow cell gamma-detection system for on-line radiochemical purity and labeling efficiency determination

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@article{07e974dd2245404b8965e3d3eaf29db4,
title = "A radio-high-performance liquid chromatography dual-flow cell gamma-detection system for on-line radiochemical purity and labeling efficiency determination",
abstract = "In this study, a method of determining radiochemical yield and radiochemical purity using radio-HPLC detection employing a dual-flow-cell system is evaluated. The dual-flow cell, consisting of a reference cell and an analytical cell, was constructed from two PEEK capillary coils to fit into the well of a NaI(Tl) detector. The radio-HPLC flow was directed from the injector to the reference cell allowing on-line detection of the total injected sample activity prior to entering the HPLC column. The radioactivity eluted from the column was then detected in the analytical cell. In this way, the sample will act as its own standard, a feature enabling on-line quantification of the processed radioactivity passing through the system. All data were acquired on-line via an analog signal from a rate meter using chromatographic software. The radiochemical yield and recovery could be simply and accurately determined by integration of the peak areas in the chromatogram obtained from the reference and analytical cells using an experimentally determined volume factor to correct for the effect of different cell volumes.",
keywords = "Astatine, Benzoates, Chromatography, High Pressure Liquid, Iodine Radioisotopes, Isotope Labeling, Limit of Detection, Radiopharmaceuticals, Technetium, Trimethyltin Compounds",
author = "S Lindegren and H Jensen and L Jacobsson",
note = "Copyright {\circledC} 2014 Elsevier B.V. All rights reserved.",
year = "2014",
month = "4",
day = "11",
doi = "10.1016/j.chroma.2014.02.043",
language = "English",
volume = "1337",
pages = "128--32",
journal = "Journal of Chromatography",
issn = "0021-9673",
publisher = "Elsevier BV",

}

RIS

TY - JOUR

T1 - A radio-high-performance liquid chromatography dual-flow cell gamma-detection system for on-line radiochemical purity and labeling efficiency determination

AU - Lindegren, S

AU - Jensen, H

AU - Jacobsson, L

N1 - Copyright © 2014 Elsevier B.V. All rights reserved.

PY - 2014/4/11

Y1 - 2014/4/11

N2 - In this study, a method of determining radiochemical yield and radiochemical purity using radio-HPLC detection employing a dual-flow-cell system is evaluated. The dual-flow cell, consisting of a reference cell and an analytical cell, was constructed from two PEEK capillary coils to fit into the well of a NaI(Tl) detector. The radio-HPLC flow was directed from the injector to the reference cell allowing on-line detection of the total injected sample activity prior to entering the HPLC column. The radioactivity eluted from the column was then detected in the analytical cell. In this way, the sample will act as its own standard, a feature enabling on-line quantification of the processed radioactivity passing through the system. All data were acquired on-line via an analog signal from a rate meter using chromatographic software. The radiochemical yield and recovery could be simply and accurately determined by integration of the peak areas in the chromatogram obtained from the reference and analytical cells using an experimentally determined volume factor to correct for the effect of different cell volumes.

AB - In this study, a method of determining radiochemical yield and radiochemical purity using radio-HPLC detection employing a dual-flow-cell system is evaluated. The dual-flow cell, consisting of a reference cell and an analytical cell, was constructed from two PEEK capillary coils to fit into the well of a NaI(Tl) detector. The radio-HPLC flow was directed from the injector to the reference cell allowing on-line detection of the total injected sample activity prior to entering the HPLC column. The radioactivity eluted from the column was then detected in the analytical cell. In this way, the sample will act as its own standard, a feature enabling on-line quantification of the processed radioactivity passing through the system. All data were acquired on-line via an analog signal from a rate meter using chromatographic software. The radiochemical yield and recovery could be simply and accurately determined by integration of the peak areas in the chromatogram obtained from the reference and analytical cells using an experimentally determined volume factor to correct for the effect of different cell volumes.

KW - Astatine

KW - Benzoates

KW - Chromatography, High Pressure Liquid

KW - Iodine Radioisotopes

KW - Isotope Labeling

KW - Limit of Detection

KW - Radiopharmaceuticals

KW - Technetium

KW - Trimethyltin Compounds

U2 - 10.1016/j.chroma.2014.02.043

DO - 10.1016/j.chroma.2014.02.043

M3 - Journal article

VL - 1337

SP - 128

EP - 132

JO - Journal of Chromatography

JF - Journal of Chromatography

SN - 0021-9673

ER -

ID: 45027868