A peptide with N-terminal histidine and C-terminal isoleucine amide (PHI) and vasoactive intestinal peptide (VIP) are copackaged in myenteric neurones of the guinea pig ileum

D V Agoston; J Fahrenkrug; J D Mikkelsen; V P Whittaker

doi:10.1016/0196-9781(89)90144-7

A peptide with N-terminal histidine and C-terminal isoleucine amide (PHI) and vasoactive intestinal peptide (VIP) are copackaged in myenteric neurones of the guinea pig ileum

D V Agoston, J Fahrenkrug, J D Mikkelsen, V P Whittaker

8 Citations (Scopus)

Abstract

When cytoplasmic extracts of the myenteric plexus of guinea pig ileum are submitted to centrifugal density gradient separation in a zonal rotor, conditions which separate storage particles containing substance P, somatostatin and VIP from each other, PHI copurifies with VIP. The two immunoreactivities cannot be separated by particle exclusion chromatography, which depends on size rather than density. It is concluded that the posttranslational cleavage of the propeptide or precursor to PHI and VIP occurs after packaging into these storage particles.

Original language	English
Journal	Peptides
Volume	10
Issue number	3
Pages (from-to)	571-3
Number of pages	3
ISSN	0196-9781
DOIs	https://doi.org/10.1016/0196-9781(89)90144-7
Publication status	Published - 1989
Externally published	Yes

Keywords

Animals
Centrifugation, Zonal
Chromatography, Gel
Guinea Pigs
Ileum/innervation
Myenteric Plexus/analysis
Neurons/analysis
Peptide PHI/analysis
Radioimmunoassay
Vasoactive Intestinal Peptide/analysis

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10.1016/0196-9781(89)90144-7

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title = "A peptide with N-terminal histidine and C-terminal isoleucine amide (PHI) and vasoactive intestinal peptide (VIP) are copackaged in myenteric neurones of the guinea pig ileum",

abstract = "When cytoplasmic extracts of the myenteric plexus of guinea pig ileum are submitted to centrifugal density gradient separation in a zonal rotor, conditions which separate storage particles containing substance P, somatostatin and VIP from each other, PHI copurifies with VIP. The two immunoreactivities cannot be separated by particle exclusion chromatography, which depends on size rather than density. It is concluded that the posttranslational cleavage of the propeptide or precursor to PHI and VIP occurs after packaging into these storage particles.",

keywords = "Animals, Centrifugation, Zonal, Chromatography, Gel, Guinea Pigs, Ileum/innervation, Myenteric Plexus/analysis, Neurons/analysis, Peptide PHI/analysis, Radioimmunoassay, Vasoactive Intestinal Peptide/analysis",

author = "Agoston, \{D V\} and J Fahrenkrug and Mikkelsen, \{J D\} and Whittaker, \{V P\}",

year = "1989",

doi = "10.1016/0196-9781(89)90144-7",

language = "English",

volume = "10",

pages = "571--3",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "3",

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TY - JOUR

T1 - A peptide with N-terminal histidine and C-terminal isoleucine amide (PHI) and vasoactive intestinal peptide (VIP) are copackaged in myenteric neurones of the guinea pig ileum

AU - Agoston, D V

AU - Fahrenkrug, J

AU - Mikkelsen, J D

AU - Whittaker, V P

PY - 1989

Y1 - 1989

N2 - When cytoplasmic extracts of the myenteric plexus of guinea pig ileum are submitted to centrifugal density gradient separation in a zonal rotor, conditions which separate storage particles containing substance P, somatostatin and VIP from each other, PHI copurifies with VIP. The two immunoreactivities cannot be separated by particle exclusion chromatography, which depends on size rather than density. It is concluded that the posttranslational cleavage of the propeptide or precursor to PHI and VIP occurs after packaging into these storage particles.

AB - When cytoplasmic extracts of the myenteric plexus of guinea pig ileum are submitted to centrifugal density gradient separation in a zonal rotor, conditions which separate storage particles containing substance P, somatostatin and VIP from each other, PHI copurifies with VIP. The two immunoreactivities cannot be separated by particle exclusion chromatography, which depends on size rather than density. It is concluded that the posttranslational cleavage of the propeptide or precursor to PHI and VIP occurs after packaging into these storage particles.

KW - Animals

KW - Centrifugation, Zonal

KW - Chromatography, Gel

KW - Guinea Pigs

KW - Ileum/innervation

KW - Myenteric Plexus/analysis

KW - Neurons/analysis

KW - Peptide PHI/analysis

KW - Radioimmunoassay

KW - Vasoactive Intestinal Peptide/analysis

UR - https://www.scopus.com/pages/publications/0024364030

U2 - 10.1016/0196-9781(89)90144-7

DO - 10.1016/0196-9781(89)90144-7

M3 - Journal article

C2 - 2780416

SN - 0196-9781

VL - 10

SP - 571

EP - 573

JO - Peptides

JF - Peptides

IS - 3

ER -

A peptide with N-terminal histidine and C-terminal isoleucine amide (PHI) and vasoactive intestinal peptide (VIP) are copackaged in myenteric neurones of the guinea pig ileum

Abstract

Keywords

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