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A novel, specific pro-urokinase complex on monocyte-like cells, detected by transglutaminase-catalyzed cross-linking

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Radiolabeled pro-urokinase plasminogen activator (pro-uPA) was cross-linked to a specific protein on the surface of human monocyte-like U937 cells in a reaction catalyzed by tissue transglutaminase. The conjugate formed with this unknown component had a much higher molecular weight (apparent M(r) 250,000-300,000) than the complex of pro-uPA and the urokinase plasminogen activator receptor (uPAR). There was a strong preference for the pro-form of uPA. The conjugate was recognized by antibodies against uPA but not by anti-uPAR antibodies. Nevertheless, the blocking of uPAR with a monoclonal antibody abolished the formation of the conjugate, thus showing a role of uPAR in this process.

Original languageEnglish
JournalF E B S Letters
Volume336
Issue number3
Pages (from-to)394-6
Number of pages3
ISSN0014-5793
Publication statusPublished - 28 Dec 1993

    Research areas

  • Cell Line, Cell Membrane, Cross-Linking Reagents, Electrophoresis, Polyacrylamide Gel, Humans, Lymphoma, Large B-Cell, Diffuse, Molecular Weight, Monocytes, Receptors, Cell Surface, Receptors, Urokinase Plasminogen Activator, Recombinant Proteins, Transglutaminases, Tumor Cells, Cultured, Urokinase-Type Plasminogen Activator

ID: 46434933