Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

A novel LC-MS/MS method to quantify eumelanin and pheomelanin and their relation to UVR sensitivity - A study on human skin biopsies

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Germline variants in oculocutaneous albinism genes and predisposition to familial cutaneous melanoma

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Conjunctival melanoma copy number alterations and correlation with mutation status, tumor features and clinical outcome

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Genetics of familial melanoma: 20 years after CDKN2A

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Increasing the complexity: new genes and new types of albinism

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. A cryptic BAP1 splice mutation in a family with uveal and cutaneous melanoma, and paraganglioma

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Effectiveness and safety of switching from originator to biosimilar adalimumab in patients with psoriasis

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Enhanced and Sustained Cutaneous Delivery of Vismodegib by Ablative Fractional Laser and Microemulsion Formulation

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

Melanin in the skin can be divided into eumelanin and pheomelanin subtypes. Simultaneous quantification of these subtypes could clarify their relation to skin type and skin cancer development. We describe a novel, sensitive liquid chromatography-tandem mass spectrometry method to quantify two eumelanin markers, pyrrole-2,3,5-tricarboxylic acid (PTCA) and pyrrole-2,3-dicarboxylic acid (PDCA), and two pheomelanin markers, thiazole-4,5-dicarboxylic acid (TDCA) and thiazole-2,4,5 tricarboxylic acid (TTCA), performed in a single run using the same biopsy. Volunteers with either Fitzpatrick skin type (FST) I/II or III/IV (n = 30) each provided a 4-mm punch biopsy from the buttock. Upon analysis, the FST I + II group had significantly less of all four melanin biomarkers (PTCA, 0.75 ng/mm2 ; PDCA, 0.08 ng/mm2 ; TTCA, 0.24 ng/mm2 ; and TDCA, 0.10 ng/mm2 ) versus the FST III + IV group (PTCA, 4.89 ng/mm2 ; PDCA, 0.22 ng/mm2 ; TTCA, 2.61 ng/mm2 ; and TDCA, 0.72 ng/mm2 ), p ≤ 0.003. We find that this new LC-MS/MS method is sensitive enough to quantify eumelanin and pheomelanin markers even in the lightest skin types.

Original languageEnglish
JournalPigment Cell & Melanoma Research
Volume32
Issue number6
Pages (from-to)809-816
Number of pages8
ISSN1755-1471
DOIs
Publication statusPublished - Nov 2019

ID: 58974622