Abstract
Ficolin-1, -2 and -3 are recognition molecules in the lectin complement pathway and form complexes with serine proteases named MASP-1, -2 and -3 and two nonenzymatic proteins. MASP-2 is the main initiator of lectin pathway activation, while ficolin-3 is the most abundant ficolin molecule in the circulation. The significance of lectin pathway complexes in the circulation is unknown. Thus, we established an assay for the measurement of circulating MASP-2/ficolin-3 complexes. A quantitative sandwich ELISA was developed for the measurement of the MASP-2/ficolin-3 complexes in serum based on monoclonal antibodies against MASP-2 for coating and anti-ficolin-3 for detection. In addition, we assessed the serum concentrations of ficolin-3 and MASP-2 and the extent of ficolin-3 mediated C4 deposition on acetylated BSA in samples from 97 healthy donors. The median concentration of MASP-2/ficolin-3 complexes was found to be 119.7 AU/ml (range: 2.9-615.5 AU/ml). Significant correlations were found between the level of MASP-2/ficolin-3 complexes and the concentration of ficolin-3 (Spearman r=0.2532, p=0.0124), and MASP-2 (Spearman r=0.4505, p
| Original language | English |
|---|---|
| Journal | Journal of Immunological Methods |
| Volume | 387 |
| Issue number | 1-2 |
| Pages (from-to) | 237-44 |
| Number of pages | 8 |
| ISSN | 0022-1759 |
| DOIs | |
| Publication status | Published - 31 Jan 2013 |
Keywords
- Antibodies, Monoclonal
- Complement C4
- Edetic Acid
- Enzyme-Linked Immunosorbent Assay
- Glycoproteins
- Humans
- Lectins
- Mannose-Binding Protein-Associated Serine Proteases
- Multiprotein Complexes
- Polymorphism, Single Nucleotide
- Reference Values
- Serum
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