A new simple and rapid dual assay for AFP and free beta hCG in screening for Down syndrome

B Nørgaard-Pedersen; H Alfthan; J Arends; C K Høgdall; S O Larsen; K Pettersson; U H Stenman; R Salonen

doi:10.1111/j.1399-0004.1994.tb03980.x

A new simple and rapid dual assay for AFP and free beta hCG in screening for Down syndrome

B Nørgaard-Pedersen, H Alfthan, J Arends, C K Høgdall, S O Larsen, K Pettersson, U H Stenman, R Salonen

15 Citations (Scopus)

Abstract

We have evaluated a simple and rapid 2-step dual-label assay (DELFIA) for alphafetoprotein (AFP) and free beta subunit of human gonadotropin (hCG beta) in second-trimester screening for Down syndrome. Based on stored serum samples from 1059 normal control pregnancies and 72 cases of Down syndrome, we have found the mean Multiple of Median (MoM) for AFP and free hCG beta to be 0.70 and 2.31, respectively. This is slightly but not significantly better than the values for the separate assay for AFP (0.76 MoM) and for intact hCG (2.11 MoM). However, the dual assay is much simpler than the separate assays and therefore prospective comparison trials should be carried out.

Original language	English
Journal	Clinical Genetics
Volume	45
Issue number	1
Pages (from-to)	1-4
Number of pages	4
ISSN	0009-9163
DOIs	https://doi.org/10.1111/j.1399-0004.1994.tb03980.x
Publication status	Published - Jan 1994
Externally published	Yes

Keywords

Chorionic Gonadotropin/blood
Down Syndrome/blood
Female
Gonadotropins/blood
Humans
Pregnancy
Prenatal Diagnosis
alpha-Fetoproteins/analysis

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10.1111/j.1399-0004.1994.tb03980.x

Cite this

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title = "A new simple and rapid dual assay for AFP and free beta hCG in screening for Down syndrome",

abstract = "We have evaluated a simple and rapid 2-step dual-label assay (DELFIA) for alphafetoprotein (AFP) and free beta subunit of human gonadotropin (hCG beta) in second-trimester screening for Down syndrome. Based on stored serum samples from 1059 normal control pregnancies and 72 cases of Down syndrome, we have found the mean Multiple of Median (MoM) for AFP and free hCG beta to be 0.70 and 2.31, respectively. This is slightly but not significantly better than the values for the separate assay for AFP (0.76 MoM) and for intact hCG (2.11 MoM). However, the dual assay is much simpler than the separate assays and therefore prospective comparison trials should be carried out.",

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T1 - A new simple and rapid dual assay for AFP and free beta hCG in screening for Down syndrome

AU - Nørgaard-Pedersen, B

AU - Alfthan, H

AU - Arends, J

AU - Høgdall, C K

AU - Larsen, S O

AU - Pettersson, K

AU - Stenman, U H

AU - Salonen, R

PY - 1994/1

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N2 - We have evaluated a simple and rapid 2-step dual-label assay (DELFIA) for alphafetoprotein (AFP) and free beta subunit of human gonadotropin (hCG beta) in second-trimester screening for Down syndrome. Based on stored serum samples from 1059 normal control pregnancies and 72 cases of Down syndrome, we have found the mean Multiple of Median (MoM) for AFP and free hCG beta to be 0.70 and 2.31, respectively. This is slightly but not significantly better than the values for the separate assay for AFP (0.76 MoM) and for intact hCG (2.11 MoM). However, the dual assay is much simpler than the separate assays and therefore prospective comparison trials should be carried out.

AB - We have evaluated a simple and rapid 2-step dual-label assay (DELFIA) for alphafetoprotein (AFP) and free beta subunit of human gonadotropin (hCG beta) in second-trimester screening for Down syndrome. Based on stored serum samples from 1059 normal control pregnancies and 72 cases of Down syndrome, we have found the mean Multiple of Median (MoM) for AFP and free hCG beta to be 0.70 and 2.31, respectively. This is slightly but not significantly better than the values for the separate assay for AFP (0.76 MoM) and for intact hCG (2.11 MoM). However, the dual assay is much simpler than the separate assays and therefore prospective comparison trials should be carried out.

KW - Chorionic Gonadotropin/blood

KW - Down Syndrome/blood

KW - Female

KW - Gonadotropins/blood

KW - Humans

KW - Pregnancy

KW - Prenatal Diagnosis

KW - alpha-Fetoproteins/analysis

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U2 - 10.1111/j.1399-0004.1994.tb03980.x

DO - 10.1111/j.1399-0004.1994.tb03980.x

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SN - 0009-9163

VL - 45

SP - 1

EP - 4

JO - Clinical Genetics

JF - Clinical Genetics

IS - 1

ER -

A new simple and rapid dual assay for AFP and free beta hCG in screening for Down syndrome

Abstract

Keywords

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