A new multiplex PCR strategy for the simultaneous determination of four genetic polymorphisms affecting HIV-1 disease progression

Thomas Birk Kristiansen; Troels Bygum Knudsen; Stine Dahl Ohlendorff; Jesper Eugen-Olsen

doi:10.1016/S0022-1759(01)00349-0

A new multiplex PCR strategy for the simultaneous determination of four genetic polymorphisms affecting HIV-1 disease progression

Thomas Birk Kristiansen, Troels Bygum Knudsen, Stine Dahl Ohlendorff, Jesper Eugen-Olsen

40 Citations (Scopus)

Abstract

The CCR5 Delta32, CCR2 64I, SDF1 3'A, and CCR5 promoter 59029 polymorphisms have been suggested to influence HIV-1 disease progression. Furthermore, the CCR5 Delta32 and the CCR2 64I polymorphisms have been associated with various other diseases. The purpose of the present study was to develop a multiplex assay for the simultaneous determination of these four polymorphisms. Results obtained with the multiplex assay were compared to results obtained by conventional RFLP-PCR and no differences were observed. The multiplex assay offers a quick tool for the determination of the CCR5 Delta32, CCR2 64I, SDF1 3'A, and CCR5 promoter 59029 A/G polymorphisms.

Original language	English
Journal	Journal of Immunological Methods
Volume	252
Issue number	1-2
Pages (from-to)	147-51
Number of pages	5
ISSN	0022-1759
DOIs	https://doi.org/10.1016/S0022-1759(01)00349-0
Publication status	Published - 2001

Keywords

Chemokine CXCL12
Chemokines, CXC
Disease Progression
HIV Infections
HIV-1
Humans
Polymerase Chain Reaction
Polymorphism, Genetic
Receptors, CCR2
Receptors, CCR5
Receptors, Chemokine

Access to Document

10.1016/S0022-1759(01)00349-0

http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T2Y-42WXG14-H-3&_cdi=4931&_user=641710&_orig=search&_coverDate=06%2F01%2F2001&_qd=1&_sk=997479998&view=c&wchp=dGLbVzz-zSkWz&md5=80a143a789eb2fe41c32625afaa66fed&ie=/sdarticle.pdf

Cite this

A new multiplex PCR strategy for the simultaneous determination of four genetic polymorphisms affecting HIV-1 disease progression. / Kristiansen, Thomas Birk; Knudsen, Troels Bygum; Ohlendorff, Stine Dahl et al.
In: Journal of Immunological Methods, Vol. 252, No. 1-2, 2001, p. 147-51.

Research output: Contribution to journal › Journal article › Research › peer-review

@article{450a77293aa542c59e005aafce7fdf2c,

title = "A new multiplex PCR strategy for the simultaneous determination of four genetic polymorphisms affecting HIV-1 disease progression",

abstract = "The CCR5 Delta32, CCR2 64I, SDF1 3'A, and CCR5 promoter 59029 polymorphisms have been suggested to influence HIV-1 disease progression. Furthermore, the CCR5 Delta32 and the CCR2 64I polymorphisms have been associated with various other diseases. The purpose of the present study was to develop a multiplex assay for the simultaneous determination of these four polymorphisms. Results obtained with the multiplex assay were compared to results obtained by conventional RFLP-PCR and no differences were observed. The multiplex assay offers a quick tool for the determination of the CCR5 Delta32, CCR2 64I, SDF1 3'A, and CCR5 promoter 59029 A/G polymorphisms.",

keywords = "Chemokine CXCL12, Chemokines, CXC, Disease Progression, HIV Infections, HIV-1, Humans, Polymerase Chain Reaction, Polymorphism, Genetic, Receptors, CCR2, Receptors, CCR5, Receptors, Chemokine",

author = "Kristiansen, \{Thomas Birk\} and Knudsen, \{Troels Bygum\} and Ohlendorff, \{Stine Dahl\} and Jesper Eugen-Olsen",

year = "2001",

doi = "10.1016/S0022-1759(01)00349-0",

language = "English",

volume = "252",

pages = "147--51",

journal = "Journal of Immunological Methods",

issn = "0022-1759",

publisher = "Elsevier BV",

number = "1-2",

}

TY - JOUR

T1 - A new multiplex PCR strategy for the simultaneous determination of four genetic polymorphisms affecting HIV-1 disease progression

AU - Kristiansen, Thomas Birk

AU - Knudsen, Troels Bygum

AU - Ohlendorff, Stine Dahl

AU - Eugen-Olsen, Jesper

PY - 2001

Y1 - 2001

N2 - The CCR5 Delta32, CCR2 64I, SDF1 3'A, and CCR5 promoter 59029 polymorphisms have been suggested to influence HIV-1 disease progression. Furthermore, the CCR5 Delta32 and the CCR2 64I polymorphisms have been associated with various other diseases. The purpose of the present study was to develop a multiplex assay for the simultaneous determination of these four polymorphisms. Results obtained with the multiplex assay were compared to results obtained by conventional RFLP-PCR and no differences were observed. The multiplex assay offers a quick tool for the determination of the CCR5 Delta32, CCR2 64I, SDF1 3'A, and CCR5 promoter 59029 A/G polymorphisms.

AB - The CCR5 Delta32, CCR2 64I, SDF1 3'A, and CCR5 promoter 59029 polymorphisms have been suggested to influence HIV-1 disease progression. Furthermore, the CCR5 Delta32 and the CCR2 64I polymorphisms have been associated with various other diseases. The purpose of the present study was to develop a multiplex assay for the simultaneous determination of these four polymorphisms. Results obtained with the multiplex assay were compared to results obtained by conventional RFLP-PCR and no differences were observed. The multiplex assay offers a quick tool for the determination of the CCR5 Delta32, CCR2 64I, SDF1 3'A, and CCR5 promoter 59029 A/G polymorphisms.

KW - Chemokine CXCL12

KW - Chemokines, CXC

KW - Disease Progression

KW - HIV Infections

KW - HIV-1

KW - Humans

KW - Polymerase Chain Reaction

KW - Polymorphism, Genetic

KW - Receptors, CCR2

KW - Receptors, CCR5

KW - Receptors, Chemokine

UR - https://www.scopus.com/pages/publications/0035371942

U2 - 10.1016/S0022-1759(01)00349-0

DO - 10.1016/S0022-1759(01)00349-0

M3 - Journal article

C2 - 11334974

SN - 0022-1759

VL - 252

SP - 147

EP - 151

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 1-2

ER -

A new multiplex PCR strategy for the simultaneous determination of four genetic polymorphisms affecting HIV-1 disease progression

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this