A new experimental model in rats for study of the pathophysiology of subarachnoid hemorrhage

Giselle F Prunell; Tiit Mathiesen; Niels-Aage Svendgaard

doi:10.1097/00001756-200212200-00034

A new experimental model in rats for study of the pathophysiology of subarachnoid hemorrhage

Giselle F Prunell, Tiit Mathiesen, Niels-Aage Svendgaard

Abstract

A new experimental model of subarachnoid hemorrhage (SAH) in rats is described. A needle was stereotaxically placed in the prechiasmatic cistern and 300, 250 or 200 microl of blood was injected manually, keeping the intracranial pressure (ICP) at the mean arterial blood pressure (MABP) level. An acceptable mortality was observed only after injection of 200 microl of blood. In this group, MABP and ICP increased immediately after SAH, but soon approached baseline levels. The subarachnoid blood was mainly distributed in the basal cisternal system and its estimated volume was about 95% of the amount injected. This new model resembles clinical SAH, is very reproducible, easy to use and seems to be a suitable model for studies of the pathophysiology of SAH.

Original language	English
Journal	NeuroReport
Volume	13
Issue number	18
Pages (from-to)	2553-6
Number of pages	4
ISSN	0959-4965
DOIs	https://doi.org/10.1097/00001756-200212200-00034
Publication status	Published - 20 Dec 2002
Externally published	Yes

Keywords

Animals
Disease Models, Animal
Male
Rats
Rats, Sprague-Dawley
Subarachnoid Hemorrhage/mortality
Wounds, Stab/mortality

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10.1097/00001756-200212200-00034

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title = "A new experimental model in rats for study of the pathophysiology of subarachnoid hemorrhage",

abstract = "A new experimental model of subarachnoid hemorrhage (SAH) in rats is described. A needle was stereotaxically placed in the prechiasmatic cistern and 300, 250 or 200 microl of blood was injected manually, keeping the intracranial pressure (ICP) at the mean arterial blood pressure (MABP) level. An acceptable mortality was observed only after injection of 200 microl of blood. In this group, MABP and ICP increased immediately after SAH, but soon approached baseline levels. The subarachnoid blood was mainly distributed in the basal cisternal system and its estimated volume was about 95% of the amount injected. This new model resembles clinical SAH, is very reproducible, easy to use and seems to be a suitable model for studies of the pathophysiology of SAH.",

keywords = "Animals, Disease Models, Animal, Male, Rats, Rats, Sprague-Dawley, Subarachnoid Hemorrhage/mortality, Wounds, Stab/mortality",

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year = "2002",

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doi = "10.1097/00001756-200212200-00034",

language = "English",

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T1 - A new experimental model in rats for study of the pathophysiology of subarachnoid hemorrhage

AU - Prunell, Giselle F

AU - Mathiesen, Tiit

AU - Svendgaard, Niels-Aage

PY - 2002/12/20

Y1 - 2002/12/20

N2 - A new experimental model of subarachnoid hemorrhage (SAH) in rats is described. A needle was stereotaxically placed in the prechiasmatic cistern and 300, 250 or 200 microl of blood was injected manually, keeping the intracranial pressure (ICP) at the mean arterial blood pressure (MABP) level. An acceptable mortality was observed only after injection of 200 microl of blood. In this group, MABP and ICP increased immediately after SAH, but soon approached baseline levels. The subarachnoid blood was mainly distributed in the basal cisternal system and its estimated volume was about 95% of the amount injected. This new model resembles clinical SAH, is very reproducible, easy to use and seems to be a suitable model for studies of the pathophysiology of SAH.

AB - A new experimental model of subarachnoid hemorrhage (SAH) in rats is described. A needle was stereotaxically placed in the prechiasmatic cistern and 300, 250 or 200 microl of blood was injected manually, keeping the intracranial pressure (ICP) at the mean arterial blood pressure (MABP) level. An acceptable mortality was observed only after injection of 200 microl of blood. In this group, MABP and ICP increased immediately after SAH, but soon approached baseline levels. The subarachnoid blood was mainly distributed in the basal cisternal system and its estimated volume was about 95% of the amount injected. This new model resembles clinical SAH, is very reproducible, easy to use and seems to be a suitable model for studies of the pathophysiology of SAH.

KW - Animals

KW - Disease Models, Animal

KW - Male

KW - Rats

KW - Rats, Sprague-Dawley

KW - Subarachnoid Hemorrhage/mortality

KW - Wounds, Stab/mortality

U2 - 10.1097/00001756-200212200-00034

DO - 10.1097/00001756-200212200-00034

M3 - Journal article

C2 - 12499866

SN - 0959-4965

VL - 13

SP - 2553

EP - 2556

JO - NeuroReport

JF - NeuroReport

IS - 18

ER -

A new experimental model in rats for study of the pathophysiology of subarachnoid hemorrhage

Abstract

Keywords

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