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Within-host microevolution of Pseudomonas aeruginosa in Italian cystic fibrosis patients

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Marvig, Rasmus Lykke ; Dolce, Daniela ; Sommer, Lea M ; Petersen, Bent ; Ciofu, Oana ; Campana, Silvia ; Molin, Søren ; Taccetti, Giovanni ; Johansen, Helle Krogh. / Within-host microevolution of Pseudomonas aeruginosa in Italian cystic fibrosis patients. I: BMC Microbiology. 2015 ; Bind 15. s. 218.

Bibtex

@article{dc43fc0ca8764435902b912e2a1e50a4,
title = "Within-host microevolution of Pseudomonas aeruginosa in Italian cystic fibrosis patients",
abstract = "BACKGROUND: Chronic infection with Pseudomonas aeruginosa is a major cause of morbidity and mortality in cystic fibrosis (CF) patients, and a more complete understanding of P. aeruginosa within-host genomic evolution, transmission, and population genomics may provide a basis for improving intervention strategies. Here, we report the first genomic analysis of P. aeruginosa isolates sampled from Italian CF patients.RESULTS: By genome sequencing of 26 isolates sampled over 19 years from four patients, we elucidated the within-host evolution of clonal lineages in each individual patient. Many of the identified mutations were located in pathoadaptive genes previously associated with host adaptation, and we correlated mutations with changes in CF-relevant phenotypes such as antibiotic resistance. In addition, the genomic analysis revealed that three patients shared the same clone. Furthermore, we compared the genomes of the Italian CF isolates to a panel of genome sequenced strains of P. aeruginosa from other countries. Isolates from two of the Italian lineages belonged to clonal complexes of P. aeruginosa that have previously been identified in Danish CF patients, and our genomic comparison showed that clonal isolates from the same country may be more distantly related than clonal isolates from different countries.CONCLUSIONS: This is the first whole-genome analysis of P. aeruginosa isolated from Italian CF patients, and together with both phenotypic and clinical information this dataset facilitates a more detailed understanding of P. aeruginosa within-host genomic evolution, transmission, and population genomics. We conclude that the evolution of the Italian lineages resembles what has been found in other countries.",
author = "Marvig, {Rasmus Lykke} and Daniela Dolce and Sommer, {Lea M} and Bent Petersen and Oana Ciofu and Silvia Campana and S{\o}ren Molin and Giovanni Taccetti and Johansen, {Helle Krogh}",
year = "2015",
doi = "10.1186/s12866-015-0563-9",
language = "English",
volume = "15",
pages = "218",
journal = "B M C Microbiology",
issn = "1471-2180",
publisher = "BioMed Central Ltd",

}

RIS

TY - JOUR

T1 - Within-host microevolution of Pseudomonas aeruginosa in Italian cystic fibrosis patients

AU - Marvig, Rasmus Lykke

AU - Dolce, Daniela

AU - Sommer, Lea M

AU - Petersen, Bent

AU - Ciofu, Oana

AU - Campana, Silvia

AU - Molin, Søren

AU - Taccetti, Giovanni

AU - Johansen, Helle Krogh

PY - 2015

Y1 - 2015

N2 - BACKGROUND: Chronic infection with Pseudomonas aeruginosa is a major cause of morbidity and mortality in cystic fibrosis (CF) patients, and a more complete understanding of P. aeruginosa within-host genomic evolution, transmission, and population genomics may provide a basis for improving intervention strategies. Here, we report the first genomic analysis of P. aeruginosa isolates sampled from Italian CF patients.RESULTS: By genome sequencing of 26 isolates sampled over 19 years from four patients, we elucidated the within-host evolution of clonal lineages in each individual patient. Many of the identified mutations were located in pathoadaptive genes previously associated with host adaptation, and we correlated mutations with changes in CF-relevant phenotypes such as antibiotic resistance. In addition, the genomic analysis revealed that three patients shared the same clone. Furthermore, we compared the genomes of the Italian CF isolates to a panel of genome sequenced strains of P. aeruginosa from other countries. Isolates from two of the Italian lineages belonged to clonal complexes of P. aeruginosa that have previously been identified in Danish CF patients, and our genomic comparison showed that clonal isolates from the same country may be more distantly related than clonal isolates from different countries.CONCLUSIONS: This is the first whole-genome analysis of P. aeruginosa isolated from Italian CF patients, and together with both phenotypic and clinical information this dataset facilitates a more detailed understanding of P. aeruginosa within-host genomic evolution, transmission, and population genomics. We conclude that the evolution of the Italian lineages resembles what has been found in other countries.

AB - BACKGROUND: Chronic infection with Pseudomonas aeruginosa is a major cause of morbidity and mortality in cystic fibrosis (CF) patients, and a more complete understanding of P. aeruginosa within-host genomic evolution, transmission, and population genomics may provide a basis for improving intervention strategies. Here, we report the first genomic analysis of P. aeruginosa isolates sampled from Italian CF patients.RESULTS: By genome sequencing of 26 isolates sampled over 19 years from four patients, we elucidated the within-host evolution of clonal lineages in each individual patient. Many of the identified mutations were located in pathoadaptive genes previously associated with host adaptation, and we correlated mutations with changes in CF-relevant phenotypes such as antibiotic resistance. In addition, the genomic analysis revealed that three patients shared the same clone. Furthermore, we compared the genomes of the Italian CF isolates to a panel of genome sequenced strains of P. aeruginosa from other countries. Isolates from two of the Italian lineages belonged to clonal complexes of P. aeruginosa that have previously been identified in Danish CF patients, and our genomic comparison showed that clonal isolates from the same country may be more distantly related than clonal isolates from different countries.CONCLUSIONS: This is the first whole-genome analysis of P. aeruginosa isolated from Italian CF patients, and together with both phenotypic and clinical information this dataset facilitates a more detailed understanding of P. aeruginosa within-host genomic evolution, transmission, and population genomics. We conclude that the evolution of the Italian lineages resembles what has been found in other countries.

U2 - 10.1186/s12866-015-0563-9

DO - 10.1186/s12866-015-0563-9

M3 - Journal article

C2 - 26482905

VL - 15

SP - 218

JO - B M C Microbiology

JF - B M C Microbiology

SN - 1471-2180

ER -

ID: 46000761