Whole-genome amplified DNA from stored dried blood spots is reliable in high resolution melting curve and sequencing analysis

Bo G Winkel; Mads Vilhelm Hollegaard; Morten S Olesen; Jesper H Svendsen; Stig Haunsø; David M Hougaard; Jacob Tfelt-Hansen

doi:10.1186/1471-2350-12-22

Whole-genome amplified DNA from stored dried blood spots is reliable in high resolution melting curve and sequencing analysis

Bo G Winkel
, Mads Vilhelm Hollegaard
, Morten S Olesen
, Jesper H Svendsen
, Stig Haunsø
, David M Hougaard
, Jacob Tfelt-Hansen

22 Citationer (Scopus)

Abstract

The use of dried blood spots (DBS) samples in genomic workup has been limited by the relative low amounts of genomic DNA (gDNA) they contain. It remains to be proven that whole genome amplified DNA (wgaDNA) from stored DBS samples, constitutes a reliable alternative to gDNA.We wanted to compare melting curves and sequencing results from wgaDNA derived from DBS samples with gDNA derived from whole blood.

Originalsprog	Engelsk
Tidsskrift	B M C Medical Genetics
Vol/bind	12
Sider (fra-til)	22
ISSN	1471-2350
DOI	https://doi.org/10.1186/1471-2350-12-22
Status	Udgivet - 2011

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10.1186/1471-2350-12-22

Citationsformater

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title = "Whole-genome amplified DNA from stored dried blood spots is reliable in high resolution melting curve and sequencing analysis",

abstract = "The use of dried blood spots (DBS) samples in genomic workup has been limited by the relative low amounts of genomic DNA (gDNA) they contain. It remains to be proven that whole genome amplified DNA (wgaDNA) from stored DBS samples, constitutes a reliable alternative to gDNA.We wanted to compare melting curves and sequencing results from wgaDNA derived from DBS samples with gDNA derived from whole blood.",

keywords = "Adult, Blood Preservation, Blood Specimen Collection, Genome, Genome, Human, Genomics, Hot Temperature, Humans, Infant, Newborn, Kv1.5 Potassium Channel, Muscle Proteins, Nucleic Acid Amplification Techniques, Phase Transition, Sodium Channels, Specimen Handling",

author = "Winkel, \{Bo G\} and Hollegaard, \{Mads Vilhelm\} and Olesen, \{Morten S\} and Svendsen, \{Jesper H\} and Stig Hauns{\o} and Hougaard, \{David M\} and Jacob Tfelt-Hansen",

year = "2011",

doi = "10.1186/1471-2350-12-22",

language = "English",

volume = "12",

pages = "22",

journal = "B M C Medical Genetics",

issn = "1471-2350",

publisher = "BioMed Central Ltd",

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TY - JOUR

T1 - Whole-genome amplified DNA from stored dried blood spots is reliable in high resolution melting curve and sequencing analysis

AU - Winkel, Bo G

AU - Hollegaard, Mads Vilhelm

AU - Olesen, Morten S

AU - Svendsen, Jesper H

AU - Haunsø, Stig

AU - Hougaard, David M

AU - Tfelt-Hansen, Jacob

PY - 2011

Y1 - 2011

N2 - The use of dried blood spots (DBS) samples in genomic workup has been limited by the relative low amounts of genomic DNA (gDNA) they contain. It remains to be proven that whole genome amplified DNA (wgaDNA) from stored DBS samples, constitutes a reliable alternative to gDNA.We wanted to compare melting curves and sequencing results from wgaDNA derived from DBS samples with gDNA derived from whole blood.

AB - The use of dried blood spots (DBS) samples in genomic workup has been limited by the relative low amounts of genomic DNA (gDNA) they contain. It remains to be proven that whole genome amplified DNA (wgaDNA) from stored DBS samples, constitutes a reliable alternative to gDNA.We wanted to compare melting curves and sequencing results from wgaDNA derived from DBS samples with gDNA derived from whole blood.

KW - Adult

KW - Blood Preservation

KW - Blood Specimen Collection

KW - Genome

KW - Genome, Human

KW - Genomics

KW - Hot Temperature

KW - Humans

KW - Infant, Newborn

KW - Kv1.5 Potassium Channel

KW - Muscle Proteins

KW - Nucleic Acid Amplification Techniques

KW - Phase Transition

KW - Sodium Channels

KW - Specimen Handling

U2 - 10.1186/1471-2350-12-22

DO - 10.1186/1471-2350-12-22

M3 - Journal article

C2 - 21306642

SN - 1471-2350

VL - 12

SP - 22

JO - B M C Medical Genetics

JF - B M C Medical Genetics

ER -

Whole-genome amplified DNA from stored dried blood spots is reliable in high resolution melting curve and sequencing analysis

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