TY - JOUR
T1 - Which follicles make the most anti-Mullerian hormone in humans?
T2 - Evidence for an abrupt decline in AMH production at the time of follicle selection
AU - Jeppesen, J V
AU - Anderson, R A
AU - Kelsey, T W
AU - Christiansen, Sofie Lindgren
AU - Kristensen, S G
AU - Jayaprakasan, K
AU - Raine-Fenning, N
AU - Campbell, B K
AU - Andersen, Claus Yding
PY - 2013/8
Y1 - 2013/8
N2 - Anti-Müllerian hormone (AMH) is exclusively produced by granulosa cells (GC) of the developing pre-antral and antral follicles, and AMH is increasingly used to assess ovarian function. It is unclear which size follicles make the most AMH (total content) and are the main contributors to circulating AMH concentrations. To determine AMH gene expression in GC (q-RT-PCR) and follicular AMH production (Elisa and RIA) in relation to follicular development, 87 follicles (3-13 mm diameter) including both GC and the corresponding follicular fluid (FF) were collected in connection with fertility preservation of human ovaries. Further, follicle number and diameter, graded in 1 mm increments, were determined by 3D ultrasound in 113 women in their natural menstrual cycle to determine follicle number and diameter in relation to circulating AMH levels. This study demonstrates for the first time a positive association between AMH gene expression in human and both total follicular fluid AMH (P
AB - Anti-Müllerian hormone (AMH) is exclusively produced by granulosa cells (GC) of the developing pre-antral and antral follicles, and AMH is increasingly used to assess ovarian function. It is unclear which size follicles make the most AMH (total content) and are the main contributors to circulating AMH concentrations. To determine AMH gene expression in GC (q-RT-PCR) and follicular AMH production (Elisa and RIA) in relation to follicular development, 87 follicles (3-13 mm diameter) including both GC and the corresponding follicular fluid (FF) were collected in connection with fertility preservation of human ovaries. Further, follicle number and diameter, graded in 1 mm increments, were determined by 3D ultrasound in 113 women in their natural menstrual cycle to determine follicle number and diameter in relation to circulating AMH levels. This study demonstrates for the first time a positive association between AMH gene expression in human and both total follicular fluid AMH (P
U2 - 10.1093/molehr/gat024
DO - 10.1093/molehr/gat024
M3 - Journal article
C2 - 23562944
SN - 1360-9947
VL - 19
SP - 519
EP - 527
JO - Molecular Human Reproduction
JF - Molecular Human Reproduction
IS - 8
ER -