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Virulent coliphages in 1-year-old children fecal samples are fewer, but more infectious than temperate coliphages

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Mathieu, A, Dion, M, Deng, L, Tremblay, D, Moncaut, E, Shah, SA, Stokholm, J, Krogfelt, KA, Schjørring, S, Bisgaard, H, Nielsen, DS, Moineau, S & Petit, M-A 2020, 'Virulent coliphages in 1-year-old children fecal samples are fewer, but more infectious than temperate coliphages' Nature Communications, bind 11, nr. 1, s. 378. https://doi.org/10.1038/s41467-019-14042-z

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Author

Mathieu, Aurélie ; Dion, Moïra ; Deng, Ling ; Tremblay, Denise ; Moncaut, Elisabeth ; Shah, Shiraz A ; Stokholm, Jakob ; Krogfelt, Karen A ; Schjørring, Susanne ; Bisgaard, Hans ; Nielsen, Dennis S ; Moineau, Sylvain ; Petit, Marie-Agnès. / Virulent coliphages in 1-year-old children fecal samples are fewer, but more infectious than temperate coliphages. I: Nature Communications. 2020 ; Bind 11, Nr. 1. s. 378.

Bibtex

@article{c8d80e7ae11940dfb2c73ba5908b782e,
title = "Virulent coliphages in 1-year-old children fecal samples are fewer, but more infectious than temperate coliphages",
abstract = "Bacteriophages constitute an important part of the human gut microbiota, but their impact on this community is largely unknown. Here, we cultivate temperate phages produced by 900 E. coli strains isolated from 648 fecal samples from 1-year-old children and obtain coliphages directly from the viral fraction of the same fecal samples. We find that 63{\%} of strains hosted phages, while 24{\%} of the viromes contain phages targeting E. coli. 150 of these phages, half recovered from strain supernatants, half from virome (73{\%} temperate and 27{\%} virulent) were tested for their host range on 75 E. coli strains isolated from the same cohort. Temperate phages barely infected the gut strains, whereas virulent phages killed up to 68{\%} of them. We conclude that in fecal samples from children, temperate coliphages dominate, while virulent ones have greater infectivity and broader host range, likely playing a role in gut microbiota dynamics.",
author = "Aur{\'e}lie Mathieu and Mo{\"i}ra Dion and Ling Deng and Denise Tremblay and Elisabeth Moncaut and Shah, {Shiraz A} and Jakob Stokholm and Krogfelt, {Karen A} and Susanne Schj{\o}rring and Hans Bisgaard and Nielsen, {Dennis S} and Sylvain Moineau and Marie-Agn{\`e}s Petit",
year = "2020",
month = "1",
day = "17",
doi = "10.1038/s41467-019-14042-z",
language = "English",
volume = "11",
pages = "378",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",

}

RIS

TY - JOUR

T1 - Virulent coliphages in 1-year-old children fecal samples are fewer, but more infectious than temperate coliphages

AU - Mathieu, Aurélie

AU - Dion, Moïra

AU - Deng, Ling

AU - Tremblay, Denise

AU - Moncaut, Elisabeth

AU - Shah, Shiraz A

AU - Stokholm, Jakob

AU - Krogfelt, Karen A

AU - Schjørring, Susanne

AU - Bisgaard, Hans

AU - Nielsen, Dennis S

AU - Moineau, Sylvain

AU - Petit, Marie-Agnès

PY - 2020/1/17

Y1 - 2020/1/17

N2 - Bacteriophages constitute an important part of the human gut microbiota, but their impact on this community is largely unknown. Here, we cultivate temperate phages produced by 900 E. coli strains isolated from 648 fecal samples from 1-year-old children and obtain coliphages directly from the viral fraction of the same fecal samples. We find that 63% of strains hosted phages, while 24% of the viromes contain phages targeting E. coli. 150 of these phages, half recovered from strain supernatants, half from virome (73% temperate and 27% virulent) were tested for their host range on 75 E. coli strains isolated from the same cohort. Temperate phages barely infected the gut strains, whereas virulent phages killed up to 68% of them. We conclude that in fecal samples from children, temperate coliphages dominate, while virulent ones have greater infectivity and broader host range, likely playing a role in gut microbiota dynamics.

AB - Bacteriophages constitute an important part of the human gut microbiota, but their impact on this community is largely unknown. Here, we cultivate temperate phages produced by 900 E. coli strains isolated from 648 fecal samples from 1-year-old children and obtain coliphages directly from the viral fraction of the same fecal samples. We find that 63% of strains hosted phages, while 24% of the viromes contain phages targeting E. coli. 150 of these phages, half recovered from strain supernatants, half from virome (73% temperate and 27% virulent) were tested for their host range on 75 E. coli strains isolated from the same cohort. Temperate phages barely infected the gut strains, whereas virulent phages killed up to 68% of them. We conclude that in fecal samples from children, temperate coliphages dominate, while virulent ones have greater infectivity and broader host range, likely playing a role in gut microbiota dynamics.

U2 - 10.1038/s41467-019-14042-z

DO - 10.1038/s41467-019-14042-z

M3 - Journal article

VL - 11

SP - 378

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

ER -

ID: 59065555