Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Vascular endothelial growth factor and mesenchymal stem cells revealed similar bone formation to allograft in a sheep model

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Comparisons of Efficacy between Autograft and Allograft on Defect Repair In Vivo in Normal and Osteoporotic Rats

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Fitness Effects of 10-Month Frequent Low-Volume Ball Game Training or Interval Running for 8-10-Year-Old School Children

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Expression of C4.4A in an In Vitro Human Tissue-Engineered Skin Model

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Self-Rated Health as a Predictor of Death after Two Years: The Importance of Physical and Mental Wellbeing Postintensive Care

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Diurnal variation of magnesium and the mineral metabolism in patients with chronic kidney disease

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Discovery of thymosin β4 as a human exerkine and growth factor

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Denosumab vs. zoledronic acid treatment in post-menopausal breast cancer: a 2-year prospective observational study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. High bone mineral density in lifelong trained female team handball players and young elite football players

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

Introduction: Mesenchymal stem cells (MSCs) and vascular endothelial growth factor (VEGF) are key factors in bone regeneration. Further stimulation should establish an enhanced cell environment optimal for vessel evolvement and hereby being able to attract bone-forming cells. The aim of this study was to generate new bone by using MSCs and VEGF, being able to stimulate growth equal to allograft.

Methods: Eight Texel/Gotland sheep had four titanium implants in a size of 10 × 12 mm inserted into bilateral distal femurs, containing a 2 mm gap. In the gap, autologous 3 × 106 MSCs seeded on hydroxyapatite (HA) granules in combination with 10 ng, 100 ng, and 500 ng VEGF release/day were added. After 12 weeks, the implant-bone blocks were harvested, embedded, and sectioned for histomorphometric analysis. Bone formation and mechanical fixation were evaluated. Blood samples were collected for the determination of bone-related biomarkers and VEGF in serum at weeks 0, 1, 4, 8, and 12.

Results: The combination of 3 × 106 MSCs with 10 ng, 100 ng, and 500 ng VEGF release/day exhibited similar amount of bone formation within the gap as allograft (P > 0.05). Moreover, no difference in mechanical fixation was observed between the groups (P > 0.05). Serum biomarkers showed no significant difference compared to baseline (all P > 0.05).

Conclusion: MSCs and VEGF exhibit significant bone regeneration, and their bone properties equal to allograft, with no systemic increase in osteogenic markers or VEGF with no visible side effects. This study indicates a possible new approach into solving the problem of insufficient allograft, in larger bone defects.

OriginalsprogEngelsk
Artikelnummer6676609
TidsskriftBioMed Research International
Vol/bind2021
ISSN2314-6133
DOI
StatusUdgivet - 2021

ID: 64874384