Variation in FCN1 affects biosynthesis of ficolin-1 and is associated with outcome of systemic inflammation

L Munthe-Fog, T Hummelshoj, Christian Le Fèvre Honoré, M E Moller, M O Skjoedt, I Palsgaard, N Borregaard, H O Madsen, P Garred

65 Citationer (Scopus)

Abstract

Ficolin-1 is a recognition molecule of the lectin complement pathway. The ficolin-1 gene FCN1 is polymorphic, but the functional and clinical consequences are unknown.The concentration of ficolin-1 in plasma and FCN1 polymorphisms in positions -1981 (rs2989727), -791 (rs28909068), -542 (rs10120023), -271 (rs28909976), -144 (rs10117466) and +7918 (rs1071583) were determined in 100 healthy individuals. FCN1 expression by isolated monocytes and granulocytes and ficolin-1 levels in monocyte culture supernatants were assessed in 21 FCN1-genotyped individuals. FCN1 polymorphisms were determined in a cohort of 251 patients with systemic inflammation. High ficolin-1 plasma levels were significantly associated with the minor alleles in position -542 and -144. These alleles were also significantly associated with high FCN1 mRNA expression. The level of ficolin-1 in culture supernatants was significantly higher in individuals homozygous for the minor alleles at positions -542 and -144. Homozygosity for these alleles was significantly associated with fatal outcome in patients with systemic inflammation. None of the other investigated polymorphisms were associated with FCN1 and ficolin-1 expression, concentration or disease outcome. Functional polymorphic sites in the promoter region of FCN1 regulate both the expression and synthesis of ficolin-1 and are associated with outcome in severe inflammation.
OriginalsprogEngelsk
TidsskriftGenes and Immunity
Vol/bind13
Udgave nummer7
Sider (fra-til)515-22
Antal sider8
ISSN1466-4879
DOI
StatusUdgivet - 2012

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