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Valproic acid modulates platelet and coagulation function ex vivo

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Bambakidis, T, Dekker, SE, Halaweish, I, Liu, B, Nikolian, VC, Georgoff, PE, Piascik, P, Li, Y, Sillesen, M & Alam, HB 2017, 'Valproic acid modulates platelet and coagulation function ex vivo', Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis, bind 28, nr. 6, s. 479-484. https://doi.org/10.1097/MBC.0000000000000626

APA

Bambakidis, T., Dekker, S. E., Halaweish, I., Liu, B., Nikolian, V. C., Georgoff, P. E., Piascik, P., Li, Y., Sillesen, M., & Alam, H. B. (2017). Valproic acid modulates platelet and coagulation function ex vivo. Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis, 28(6), 479-484. https://doi.org/10.1097/MBC.0000000000000626

CBE

MLA

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Author

Bambakidis, Ted ; Dekker, Simone E ; Halaweish, Ihab ; Liu, Baoling ; Nikolian, Vahagn C ; Georgoff, Patrick E ; Piascik, Patryk ; Li, Yongqing ; Sillesen, Martin ; Alam, Hasan B. / Valproic acid modulates platelet and coagulation function ex vivo. I: Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis. 2017 ; Bind 28, Nr. 6. s. 479-484.

Bibtex

@article{c7cb5b90245a44a3bedbb4888b4c2434,
title = "Valproic acid modulates platelet and coagulation function ex vivo",
abstract = ": Trauma-induced coagulopathy is associated with adverse patient outcome. Animal models demonstrate that histone deacetylase inhibitors, such as valproic acid (VPA), improve survival following injury. While in-vivo data suggest that improved survival may in part be because of an attenuation of coagulopathy, it remains unknown whether this is a direct effect of the drug, or the establishment of an overall prosurvival phenotype. We thus conducted an ex-vivo experiment to determine if VPA has an effect on coagulation and platelet function. Ten swine were subjected to traumatic brain injury (TBI) and hemorrhagic shock (HS). Blood samples were drawn prior to TBI+HS insult (Healthy group) and 2 h following TBI+HS (Shock group). Samples were incubated with VPA or vehicle controls for 1 h. Platelet aggregation was analyzed via impedance aggregometry and coagulation was measured using thromboelastography. Addition of VPA to the healthy blood did not affect platelet aggregation or coagulation parameters. In shock blood, incubation with VPA significantly reduced collagen-(P = 0.050), arachidonic acid-(P = 0.005), and adenosine diphosphate-(P = 0.023) induced platelet aggregation. VPA also significantly increased the clot strength (P = 0.002) and clot formation rate (P = 0.011). This is the first study to investigate the effect of VPA on platelet function ex vivo. Our results suggest that VPA has no effect on normal blood, but it decreases platelet activation and improves clot dynamics (strength and rate of formation) in blood from shocked animals. This suggests that VPA is capable of exerting a selective platelet sparing effect while enhancing the clot integrity.",
keywords = "Journal Article",
author = "Ted Bambakidis and Dekker, {Simone E} and Ihab Halaweish and Baoling Liu and Nikolian, {Vahagn C} and Georgoff, {Patrick E} and Patryk Piascik and Yongqing Li and Martin Sillesen and Alam, {Hasan B}",
year = "2017",
month = sep,
doi = "10.1097/MBC.0000000000000626",
language = "English",
volume = "28",
pages = "479--484",
journal = "Blood Coagulation and Fibrinolysis",
issn = "0957-5235",
publisher = "Lippincott Williams & Wilkins",
number = "6",

}

RIS

TY - JOUR

T1 - Valproic acid modulates platelet and coagulation function ex vivo

AU - Bambakidis, Ted

AU - Dekker, Simone E

AU - Halaweish, Ihab

AU - Liu, Baoling

AU - Nikolian, Vahagn C

AU - Georgoff, Patrick E

AU - Piascik, Patryk

AU - Li, Yongqing

AU - Sillesen, Martin

AU - Alam, Hasan B

PY - 2017/9

Y1 - 2017/9

N2 - : Trauma-induced coagulopathy is associated with adverse patient outcome. Animal models demonstrate that histone deacetylase inhibitors, such as valproic acid (VPA), improve survival following injury. While in-vivo data suggest that improved survival may in part be because of an attenuation of coagulopathy, it remains unknown whether this is a direct effect of the drug, or the establishment of an overall prosurvival phenotype. We thus conducted an ex-vivo experiment to determine if VPA has an effect on coagulation and platelet function. Ten swine were subjected to traumatic brain injury (TBI) and hemorrhagic shock (HS). Blood samples were drawn prior to TBI+HS insult (Healthy group) and 2 h following TBI+HS (Shock group). Samples were incubated with VPA or vehicle controls for 1 h. Platelet aggregation was analyzed via impedance aggregometry and coagulation was measured using thromboelastography. Addition of VPA to the healthy blood did not affect platelet aggregation or coagulation parameters. In shock blood, incubation with VPA significantly reduced collagen-(P = 0.050), arachidonic acid-(P = 0.005), and adenosine diphosphate-(P = 0.023) induced platelet aggregation. VPA also significantly increased the clot strength (P = 0.002) and clot formation rate (P = 0.011). This is the first study to investigate the effect of VPA on platelet function ex vivo. Our results suggest that VPA has no effect on normal blood, but it decreases platelet activation and improves clot dynamics (strength and rate of formation) in blood from shocked animals. This suggests that VPA is capable of exerting a selective platelet sparing effect while enhancing the clot integrity.

AB - : Trauma-induced coagulopathy is associated with adverse patient outcome. Animal models demonstrate that histone deacetylase inhibitors, such as valproic acid (VPA), improve survival following injury. While in-vivo data suggest that improved survival may in part be because of an attenuation of coagulopathy, it remains unknown whether this is a direct effect of the drug, or the establishment of an overall prosurvival phenotype. We thus conducted an ex-vivo experiment to determine if VPA has an effect on coagulation and platelet function. Ten swine were subjected to traumatic brain injury (TBI) and hemorrhagic shock (HS). Blood samples were drawn prior to TBI+HS insult (Healthy group) and 2 h following TBI+HS (Shock group). Samples were incubated with VPA or vehicle controls for 1 h. Platelet aggregation was analyzed via impedance aggregometry and coagulation was measured using thromboelastography. Addition of VPA to the healthy blood did not affect platelet aggregation or coagulation parameters. In shock blood, incubation with VPA significantly reduced collagen-(P = 0.050), arachidonic acid-(P = 0.005), and adenosine diphosphate-(P = 0.023) induced platelet aggregation. VPA also significantly increased the clot strength (P = 0.002) and clot formation rate (P = 0.011). This is the first study to investigate the effect of VPA on platelet function ex vivo. Our results suggest that VPA has no effect on normal blood, but it decreases platelet activation and improves clot dynamics (strength and rate of formation) in blood from shocked animals. This suggests that VPA is capable of exerting a selective platelet sparing effect while enhancing the clot integrity.

KW - Journal Article

U2 - 10.1097/MBC.0000000000000626

DO - 10.1097/MBC.0000000000000626

M3 - Journal article

C2 - 28230635

VL - 28

SP - 479

EP - 484

JO - Blood Coagulation and Fibrinolysis

JF - Blood Coagulation and Fibrinolysis

SN - 0957-5235

IS - 6

ER -

ID: 52410970